Imai Y, Kohsaka S
Department of Neurochemistry, National Institute of Neuroscience, Japan.
Eur J Biochem. 1995 Sep 1;232(2):327-34.
We report here the isolation and characterization of the entire rat annexin V gene, which is shown to span about 40 kbp, and to consist of 14 exons, 13 introns and two promoters. Previous studies found that both human and chicken annexin V genes bear 13 exons and one promoter, which possess no TATA sequence or CCAAT box, and have a high GC content. In the present study, we found that one of the promoters for rat annexin V shares these features, which are typical among promoters for housekeeping genes, and that this promoter, as expected, exerted its transcription in all of tissues we examined. In contrast, another promoter was found to possess not only a TATA box but also a glucocorticoid-responsive element, and was shown to express messages in a relatively limited number of tissues, including brain. We also demonstrated the presence of at least four species of rat annexin V transcripts that were generated by distinct transcription initiation and alternative splicing.
我们在此报告大鼠膜联蛋白V基因全长的分离及特性分析，该基因约跨越40千碱基对，由14个外显子、13个内含子和两个启动子组成。先前的研究发现，人类和鸡的膜联蛋白V基因均有13个外显子和一个启动子，它们没有TATA序列或CCAAT框，且GC含量高。在本研究中，我们发现大鼠膜联蛋白V的一个启动子具有这些特征，这在管家基因启动子中很典型，并且正如预期的那样，该启动子在我们检测的所有组织中均发挥转录作用。相比之下，另一个启动子不仅具有TATA框，还具有糖皮质激素反应元件，并且在包括脑在内的相对有限的组织中表达信使RNA。我们还证明了存在至少四种由不同转录起始和可变剪接产生的大鼠膜联蛋白V转录本。
