Drize N, Chertkov J, Zander A
Hematological Scientific Center, Russian Academy of Medical Science, Moscow.
Exp Hematol. 1995 Oct;23(11):1180-6.
A significant increase in the hematopoietic stem cell (HSC) concentration has been observed in the peripheral blood and spleen of mice treated with rhG-CSF alone or with a combination of rhG-CSF plus rrSCF. The longer the duration of cytokine treatment, the higher the stimulatory effect on stem cell mobilization. In addition, cytokine administration led to a reduced stem cell concentration in the bone marrow. The total amount of HSCs in the body did not change following cytokine administration. These data support the theory that stem cells are mobilized from the bone marrow into circulation, as opposed to expanding in blood and spleen. In sharp contrast to rhG-CSF alone, a combination of rhG-CSF plus rrSCF produced a strong increase in the self-maintenance ability of peripheral blood day 11 colony-forming units-spleen (CFU-S-11). After 10 days of cytokine treatment, long-term culture initiating cells (LTC-IC) were seen in the peripheral blood; in normal mice, the content of LTC-IC in the blood was below the detection level. The activation of stromal progenitors (cells capable of transferring the hematopoietic microenvironment) by cytokine treatment was observed here for the first time. The results suggest that a combination of rhG-CSF plus rrSCF is more effective than rhG-CSF alone in obtaining a large amount of transplantable HSCs.
在单独使用重组人粒细胞集落刺激因子（rhG-CSF）或rhG-CSF与重组大鼠干细胞因子（rrSCF）联合治疗的小鼠外周血和脾脏中，已观察到造血干细胞（HSC）浓度显著增加。细胞因子治疗的持续时间越长，对干细胞动员的刺激作用就越高。此外，细胞因子给药导致骨髓中干细胞浓度降低。细胞因子给药后，体内造血干细胞的总量没有变化。这些数据支持了干细胞从骨髓动员到循环系统的理论，而不是在血液和脾脏中扩增。与单独使用rhG-CSF形成鲜明对比的是，rhG-CSF与rrSCF联合使用使外周血第11天脾集落形成单位（CFU-S-11）的自我维持能力大幅提高。细胞因子治疗10天后，在外周血中发现了长期培养起始细胞（LTC-IC）；在正常小鼠中，血液中LTC-IC的含量低于检测水平。在此首次观察到细胞因子治疗对基质祖细胞（能够传递造血微环境的细胞）的激活作用。结果表明，rhG-CSF与rrSCF联合使用在获得大量可移植造血干细胞方面比单独使用rhG-CSF更有效。
