Sasaki Y, Ishii K, Nunoki K, Yamagishi T, Taira N
Department of Pharmacology, Tohoku University School of Medicine, Sendai, Japan.
FEBS Lett. 1995 Sep 18;372(1):20-4. doi: 10.1016/0014-5793(95)00954-8.
We have isolated a cDNA coding for a delayed rectifier K+ channel (RBKV1.5) from rabbit heart. The amino acid sequence of RBKV1.5 displays a homology to that of other K+ channels of Kv1.5 class. Overall amino acid identity between RBKV1.5 channel and Kv1.5 channel of other species is about 85%. RNA blot analysis revealed the expression of the primary transcript in various rabbit tissues, at the highest level in both the atrium and ventricle. When expressed in Xenopus oocytes, RBKV1.5 current showed a delayed rectifier type characteristics, which was converted to rapidly inactivating currents upon coexpression with a beta subunit.
我们从兔心脏中分离出了一个编码延迟整流钾离子通道（RBKV1.5）的cDNA。RBKV1.5的氨基酸序列与Kv1.5类的其他钾离子通道具有同源性。RBKV1.5通道与其他物种的Kv1.5通道之间的总体氨基酸同一性约为85%。RNA印迹分析显示初级转录本在兔的各种组织中均有表达，在心房和心室中的表达水平最高。当在非洲爪蟾卵母细胞中表达时，RBKV1.5电流呈现出延迟整流型特征，与β亚基共表达时则转变为快速失活电流。
