Grozdanovic Z, Nakos G, Mayer B, Gossrau R
Department of Anatomy, Free University of Berlin, Germany.
Folia Histochem Cytobiol. 1995;33(1):11-8.
Results obtained with the conventional nitro blue tetrazolium salt method for the visualization of the NADPH-diaphorase (NADPH-d) activity of nitric oxide synthase (NOS) are not specific for this particular enzyme, since this activity represents only a fraction of the total cellular NADPH-d pool. Therefore, the standard NADPH-d procedure was modified by performing the incubation in the presence of formaldehyde. Parallel application of the modified NADPH-d staining technique and the indirect immunofluorescence using an antibody against the neuronal isoenzyme (nNOS) on rat, mouse and guinea-pig tissues showed a correlation between histochemical and immunocytochemical staining. It can thus be concluded that the modified NADPH-d procedure allows for a more specific detection of the histochemical nNOS activity than the conventional method.
用传统的硝基蓝四氮唑盐法来显示一氧化氮合酶（NOS）的NADPH-黄递酶（NADPH-d）活性所获得的结果，对于这种特定的酶来说并不具有特异性，因为这种活性仅占细胞总NADPH-d库的一小部分。因此，通过在甲醛存在的情况下进行孵育，对标准的NADPH-d程序进行了修改。在大鼠、小鼠和豚鼠组织上，将改良的NADPH-d染色技术与使用抗神经元同工酶（nNOS）抗体的间接免疫荧光法并行应用，结果显示组织化学染色和免疫细胞化学染色之间存在相关性。因此可以得出结论，与传统方法相比，改良的NADPH-d程序能够更特异性地检测组织化学nNOS活性。
