Dixon B, van Erp S H, Rodrigues P N, Egberts E, Stet R J
Department of Experimental Animal Morphology and Cell Biology, Wageningen Agricultural University, The Netherlands.
Dev Comp Immunol. 1995 Mar-Apr;19(2):109-33. doi: 10.1016/0145-305x(94)00056-l.
The advent of polymerase chain reaction technology has provoked a large amount of progress in the field of fish major histocompatibility complex (MHC) research. Many new teleost sequences have been reported in the last four years, including representatives of all classes of MHC genes. While the intron-exon structure of teleost MHC genes is now becoming clear, the organisation of the genes within the teleost MHC is still unclear. The sequences reported to date have been used for phylogenetic analysis and, due to their evolutionary position, are discussed in relation to hypotheses regarding the origin of the MHC. Teleost MHC gene sequences are also examined to see if conserved features of the both the nucleotide and amino acid sequences of higher vertebrate MHC genes are present. Differences in these features will reflect functional differences between teleost and mammalian MHC genes and may also have evolutionary implications.
聚合酶链反应技术的出现推动了鱼类主要组织相容性复合体（MHC）研究领域的大量进展。在过去四年里，已经报道了许多新的硬骨鱼序列，包括所有MHC基因类别的代表。虽然硬骨鱼MHC基因的内含子-外显子结构现在正变得清晰，但硬骨鱼MHC内基因的组织方式仍不清楚。迄今为止报道的序列已用于系统发育分析，并且由于它们的进化位置，在关于MHC起源的假说背景下进行了讨论。还对硬骨鱼MHC基因序列进行了研究，以查看是否存在高等脊椎动物MHC基因核苷酸和氨基酸序列的保守特征。这些特征的差异将反映硬骨鱼和哺乳动物MHC基因之间的功能差异，也可能具有进化意义。
