Ciccotosto G D, McLeish A, Hardy K J, Shulkes A
Department of Surgery, University of Melbourne, Victoria, Australia.
Gastroenterology. 1995 Oct;109(4):1142-53. doi: 10.1016/0016-5085(95)90572-3.
BACKGROUND & AIMS: The relationship between gastrin and the development of colorectal carcinoma (CRC) remains controversial. Problems with previous studies include failure to measure all forms of gastrin, lack of comparison between stored and secreted gastrin, and not controlling for Helicobacter pylori infection (a known cause of hypergastrinemia). The aim of this study was to quantify progastrin and progastrin-derived peptides in the resected tumor and plasma of patients with CRC and in the antrum and plasma of normal subjects.
Four region-specific gastrin antisera were used to measure progastrin, glycine-extended gastrin, amidated gastrin, and total gastrin.
Progastrin, amidated gastrin, total gastrin, and glycine-extended gastrin were detected in 100%, 69%, 56%, and 44% of tumors, respectively (n = 32). When allowing for H. pylori infection, circulating amidated gastrin levels were not significantly elevated in patients with CRC. However, compared with control H. pylori-positive and H. pylori-negative subjects, fasting plasma total gastrin levels were increased in H. pylori-positive (5.2-fold) and H. pylori-negative (2.3-fold) patients with CRC.
Gastrin or its processing intermediates are present in a high proportion of CRCs. Nonamidated gastrin levels are elevated in the circulation of patients with CRC regardless of H. pylori status. We conclude that gastrin should continue to be assessed as a circulating or autocrine growth factor in the development of CRC.
胃泌素与结直肠癌（CRC）发生发展之间的关系仍存在争议。既往研究存在的问题包括未能检测所有形式的胃泌素、未对储存型和分泌型胃泌素进行比较，以及未控制幽门螺杆菌感染（已知的高胃泌素血症病因）。本研究旨在对CRC患者切除肿瘤组织和血浆以及正常受试者胃窦组织和血浆中的胃泌素原及胃泌素原衍生肽进行定量分析。
使用四种区域特异性胃泌素抗血清检测胃泌素原、甘氨酸延伸型胃泌素、酰胺化胃泌素和总胃泌素。
在32例肿瘤中，分别有100%、69%、56%和44%检测到胃泌素原、酰胺化胃泌素、总胃泌素和甘氨酸延伸型胃泌素。考虑到幽门螺杆菌感染因素后，CRC患者循环中酰胺化胃泌素水平无显著升高。然而，与幽门螺杆菌阳性和阴性的对照受试者相比，幽门螺杆菌阳性（5.2倍）和阴性（2.3倍）的CRC患者空腹血浆总胃泌素水平均升高。
胃泌素或其加工中间体在很大比例的CRC中存在。无论幽门螺杆菌感染状态如何，CRC患者循环中非酰胺化胃泌素水平均升高。我们得出结论，在CRC发生发展过程中，胃泌素仍应作为循环或自分泌生长因子进行评估。
