Inoue H, Mori M, Honda M, Li J, Shibuta K, Mimori K, Ueo H, Akiyoshi T
Department of Surgery, Kyushu University, Beppu, Japan.
Gastroenterology. 1995 Nov;109(5):1522-5. doi: 10.1016/0016-5085(95)90639-8.
BACKGROUND & AIMS: The genes MAGE-1 and MAGE-3 both encode melanoma peptide antigens recognized by major histocompatibility complex-restricted cytotoxic T lymphocytes. The antigens may be a target for immunotherapy. There is, however, little information on the expression of these genes in gastric carcinomas. Therefore, the expression of MAGE genes in gastric carcinomas was evaluated.
The expression of MAGE-1, MAGE-2, and MAGE-3 genes in tumors and corresponding normal tissue specimens was studied using a reverse-transcription polymerase chain reaction. The results were analyzed according to clinicopathologic factors of the tumor.
In the 68 gastric carcinomas studied, MAGE-1, MAGE-2, and MAGE-3 messenger RNA were detected in 41%, 31%, and 38%, respectively. Fifty percent of the gastric carcinomas expressed at least one of the MAGE genes. Messenger RNA for the three MAGE proteins was not detected in normal gastric tissue. MAGE gene expression in gastric carcinomas was not associated with a significant clincopathology of the tumor. However, gene expression was lower in mucinous carcinomas (3 of 10).
MAGE-1, MAGE-2, and MAGE-3 are expressed in a high percentage of gastric carcinomas. These tumor rejection antigens may provide tumor-specific targets for immunotherapy.
MAGE-1和MAGE-3基因均编码黑色素瘤肽抗原，这些抗原可被主要组织相容性复合体限制的细胞毒性T淋巴细胞识别。这些抗原可能是免疫治疗的靶点。然而，关于这些基因在胃癌中的表达情况知之甚少。因此，我们对胃癌中MAGE基因的表达进行了评估。
采用逆转录聚合酶链反应研究肿瘤及相应正常组织标本中MAGE-1、MAGE-2和MAGE-3基因的表达。根据肿瘤的临床病理因素分析结果。
在研究的68例胃癌中，MAGE-1、MAGE-2和MAGE-3信使核糖核酸的检测率分别为41%、31%和38%。50%的胃癌表达至少一种MAGE基因。正常胃组织中未检测到这三种MAGE蛋白的信使核糖核酸。胃癌中MAGE基因的表达与肿瘤的显著临床病理特征无关。然而，黏液癌中的基因表达较低（10例中有3例）。
MAGE-1、MAGE-2和MAGE-3在高比例的胃癌中表达。这些肿瘤排斥抗原可能为免疫治疗提供肿瘤特异性靶点。
