牙龈卟啉单胞菌脂多糖刺激对小鼠B淋巴细胞酪氨酸蛋白磷酸化的影响
Tyrosine protein phosphorylation in murine B lymphocytes by stimulation with lipopolysaccharide from Porphyromonas gingivalis.
作者信息
Kimura S, Koga T, Fujiwara T, Kontani M, Shintoku K, Kaya H, Hamada S
机构信息
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
出版信息
FEMS Microbiol Lett. 1995 Jul 15;130(1):1-6. doi: 10.1016/0378-1097(95)00175-5.
The molecular effect of lipopolysaccharides (LPS) from porphyromonas gingivalis as well as Escherichia coli on the tyrosine protein phosphorylation in the splenic B lymphocytes from LPS-responsive C3H/HeN and LPS-hyporesponsive C3H/HeJ mice was examined. P. gingivalis LPS induced tyrosine phosphorylation of selected membrane proteins that included the phosphoproteins with apparent molecular masses of 24.8 kDa and 26.0 kDa (p24.8 and p26.) in the B lymphocytes from both strains of mice, while E. coli LPS induced p24.8 and p26.0 in C3H/HeN B Lymphocytes only. These findings suggest that through the same tyrosine phosphorylation pathway as observed in C3H/HeN B lymphocytes, P. gingivalis LPS induced the activation of C3H/HeJ B lymphocytes in which a trigger signal by E. coli LPS could not be transduced to initiate tyrosine protein phosphorylation.
研究了牙龈卟啉单胞菌和大肠杆菌的脂多糖（LPS）对LPS反应性C3H/HeN小鼠和LPS低反应性C3H/HeJ小鼠脾B淋巴细胞中酪氨酸蛋白磷酸化的分子效应。牙龈卟啉单胞菌LPS在两种小鼠品系的B淋巴细胞中均诱导了选定膜蛋白的酪氨酸磷酸化，这些膜蛋白包括表观分子量为24.8 kDa和26.0 kDa的磷蛋白（p24.8和p26），而大肠杆菌LPS仅在C3H/HeN B淋巴细胞中诱导p24.8和p26.0。这些发现表明，牙龈卟啉单胞菌LPS通过与C3H/HeN B淋巴细胞中观察到的相同酪氨酸磷酸化途径，诱导了C3H/HeJ B淋巴细胞的激活，而大肠杆菌LPS的触发信号无法在其中转导以启动酪氨酸蛋白磷酸化。
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