人类水通道（AQP3）基因的结构与染色体定位
Structure and chromosomal localization of a human water channel (AQP3) gene.
作者信息
Ishibashi K, Sasaki S, Saito F, Ikeuchi T, Marumo F
机构信息
Second Department of Internal Medicine, School of Medicine, Tokyo, Japan.
出版信息
Genomics. 1995 May 20;27(2):352-4. doi: 10.1006/geno.1995.1055.
A cDNA encoding rat AQP3, a water channel and a member of the MIP family, that is expressed predominantly in kidney medulla and colon was cloned recently. To determine the structure, tissue distribution, and chromosomal localization of the human AQP3 gene, we screened a human kidney cDNA library with rat AQP3 probe and isolated a cDNA coding for human AQP3 protein. The deduced amino acid sequence of human AQP3 was 91% identical to rat AQP3. Human AQP3 mRNA was expressed in colon, kidney, liver, pancreas, lung, peripheral leukocytes, spleen, and prostate. The human AQP3 gene was mapped to 7q36.2-q36.3 by chromosome fluorescence in situ hybridization.
最近克隆了一个编码大鼠水通道蛋白3（AQP3）的cDNA，它是主要在肾髓质和结肠中表达的水通道，属于主要内在蛋白（MIP）家族成员。为了确定人类AQP3基因的结构、组织分布和染色体定位，我们用大鼠AQP3探针筛选了人类肾脏cDNA文库，并分离出一个编码人类AQP3蛋白的cDNA。推导的人类AQP3氨基酸序列与大鼠AQP3的一致性为91%。人类AQP3 mRNA在结肠、肾脏、肝脏、胰腺、肺、外周血白细胞、脾脏和前列腺中表达。通过染色体荧光原位杂交将人类AQP3基因定位到7q36.2-q36.3。
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