Paradee W, Mullins C, He Z, Glover T, Wilke C, Opalka B, Schutte J, Smith D I
Department of Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Genomics. 1995 May 20;27(2):358-61. doi: 10.1006/geno.1995.1057.
The common fragile site at 3p14.2 (FRA3B) has been described as the most active fragile site in the human genome. This locus may predispose chromosome 3 to specific losses due to deletions and translocations that have been associated with several malignancies, including hereditary renal cell carcinoma. We have previously described induction of breakage around FRA3B using aphidicolin in a somatic cell hybrid whose only human component was a single intact chromosome 3. That work led to the isolation of hybrids with breakpoints in the 3p13-p21.1 region with loss of all sequences distal to their respective breakpoints. In this report we describe the further characterization of the breakpoints in many of these cell lines using newly available molecular markers. We also report the identification of YAC clones that span the breakpoints present in many of these hybrids.
位于3p14.2的常见脆性位点（FRA3B）被认为是人类基因组中最活跃的脆性位点。由于与包括遗传性肾细胞癌在内的多种恶性肿瘤相关的缺失和易位，该基因座可能使3号染色体易于发生特定缺失。我们之前曾描述过，在一个仅含一条完整人类3号染色体的体细胞杂种中，使用阿非科林诱导FRA3B周围的断裂。这项工作导致分离出了在3p13 - p21.1区域具有断点且其各自断点远端所有序列均缺失的杂种。在本报告中，我们使用新获得的分子标记描述了许多这些细胞系中断点的进一步特征。我们还报告了跨越许多这些杂种中存在的断点的酵母人工染色体（YAC）克隆的鉴定。
