Cohen L Y, Parant M A
CNRS, Laboratoire Immunopharmacologie, Centre des Cordeliers, Paris, France.
Immunol Lett. 1995 Mar;45(3):210-4. doi: 10.1016/0165-2478(95)00007-r.
The murine pre-B cell line 70Z/3 responds to lipopolysaccharide (LPS), interleukin-1 (IL-1) or interferon-gamma (IGN gamma) by kappa gene transcription and expression of surface IgM (sIg). We found that muramyl dipeptide (MDP), a synthetic immunoadjuvant analog of a bacterial membrane structure, produced a weak increase in the number of sIg-positive 70Z/3 cells as measured by immunofluorescence staining. This number was significantly increased after exposure to MDP. Moreover, when MDP was used in combination with LPS, IL-1 or IFN gamma, an enhancement of sIg expression was observed showing an early influence of MDP in the presence of a second stimulant. Unexpectedly, two adjuvant-active analogs of MDP did not share its capacity to stimulate differentiation of the cell line when used alone or associated with other agents, indicating that adjuvanticity of MDP was not the only requirement. Two other products of bacterial origin, a Staphylococcus aureus cell extract (SAC) and the Toxic Shock Syndrome Toxin TSST-1 could neither enhance the kappa gene expression in 70Z/3 cells nor increase the MDP effect. The stimulating effect displayed by MDP could by related to NF-kappa B activation.
鼠前B细胞系70Z/3通过κ基因转录和表面IgM（sIg）的表达来响应脂多糖（LPS）、白细胞介素-1（IL-1）或干扰素-γ（IFNγ）。我们发现，胞壁酰二肽（MDP），一种细菌膜结构的合成免疫佐剂类似物，通过免疫荧光染色检测，能使sIg阳性的70Z/3细胞数量略有增加。暴露于MDP后，这个数量显著增加。此外，当MDP与LPS、IL-1或IFNγ联合使用时，观察到sIg表达增强，表明在存在第二种刺激物的情况下，MDP有早期影响。出乎意料的是，MDP的两种具有佐剂活性的类似物在单独使用或与其他试剂联合使用时，并不具备刺激该细胞系分化的能力，这表明MDP的佐剂活性并非唯一的必要条件。另外两种细菌来源的产物，金黄色葡萄球菌细胞提取物（SAC）和中毒性休克综合征毒素TSST-1，既不能增强70Z/3细胞中的κ基因表达，也不能增强MDP的作用。MDP所显示的刺激作用可能与NF-κB激活有关。
