Tang J, Dobson C, McManus D P
Department of Parasitology, University of Queensland, St Lucia, Australia.
Int J Parasitol. 1995 Jul;25(7):847-52. doi: 10.1016/0020-7519(94)00206-4.
Antigens from Heligmosomoides polygyrus, which had been passaged selectively for over 40 generations through naive (Hpn) and immune (Hpa) mice, were extracted as whole worm (WWA) and membrane bound antigens (MBA), soluble adult worm homogenates (AWH), and excretory/secretory (ES) products. The antigen complexes were separated by SDS-PAGE, and 2-dimensional electrophoresis and assayed by western blot. Qualitative and quantitative differences were observed between profiles and antigenic reactivity of the constituents from selected parasites, which reflected their genetic heterogeneity. The survival of Hpa parasite phenotypes was improved compared with that of their Hpn counterparts in homologous strains of immunized mice but this did not correlate strongly with the antigenic differences observed. Three small molecules at 18, 21 and 23 kDa, respectively, dominated the somatic and ES components of all worms but they were of low immunogenicity during natural infection in mice and after vaccination in rabbits; and their role in immunomodulation is discussed.
从经选择性传代40多代的多枝背带线虫抗原中提取全虫抗原（WWA）、膜结合抗原（MBA）、可溶性成虫匀浆（AWH）以及排泄/分泌（ES）产物，传代过程分别通过未感染过的小鼠（Hpn）和免疫过的小鼠（Hpa）。抗原复合物经SDS - PAGE分离、二维电泳，并通过蛋白质印迹法进行检测。观察到所选寄生虫成分的图谱和抗原反应性存在定性和定量差异，这反映了它们的遗传异质性。在免疫小鼠的同源品系中，Hpa寄生虫表型的存活率比Hpn对应物有所提高，但这与观察到的抗原差异没有很强的相关性。分别为18 kDa、21 kDa和23 kDa的三个小分子在所有蠕虫的体细胞和ES成分中占主导地位，但在小鼠自然感染期间和兔子接种疫苗后，它们的免疫原性较低；文中还讨论了它们在免疫调节中的作用。
