Ishikawa Y, Tokunaga K, Kashiwase K, Akaza T, Tadokoro K, Juji T
Department of Research, Japanese Red Cross Central Blood Center, Tokyo.
Hum Immunol. 1995 Apr;42(4):315-8. doi: 10.1016/0198-8859(94)00119-b.
A PCR-SBT method using genomic DNA for HLA-A2 alleles was established. To achieve specific amplification for detecting a single base difference between A2 alleles and other HLA-A alleles, a primer having one extra mismatch at the second position from its 3'-end was designed. The primer exhibited a high specificity with annealing temperatures from 64 degrees C to 68 degrees C. Thirty-eight Japanese samples were screened using this method. The majority of Japanese A2 antigens were coded by A0201. A0206 and A0207 were observed at relatively high frequencies. Serologically defined split antigens, A2S and A2AK, which we have recently identified, corresponded to A0203 and A0210, respectively. Moreover, A0207 was strongly associated with B46 and DR8.1.
建立了一种使用基因组DNA检测HLA - A2等位基因的PCR - SBT方法。为实现特异性扩增以检测A2等位基因与其他HLA - A等位基因之间的单个碱基差异，设计了一种在其3'端第二个位置具有一个额外错配的引物。该引物在64℃至68℃的退火温度下表现出高特异性。使用此方法对38个日本样本进行了筛查。大多数日本A2抗原由A0201编码。观察到A0206和A0207的频率相对较高。我们最近鉴定的血清学定义的裂解抗原A2S和A2AK分别对应于A0203和A0210。此外，A0207与B46和DR8.1密切相关。
