Horie H, Yokogoshi Y, Tsuyuguchi M, Saito S
First Department of Internal Medicine, School of Medicine, University of Tokushima.
Jpn J Cancer Res. 1995 Aug;86(8):737-42. doi: 10.1111/j.1349-7006.1995.tb02462.x.
We studied 43 thyroid tumors including 5 adenomatous goiters, 7 follicular adenomas, 22 papillary carcinomas, and 9 medullary carcinomas with regard to the presence of point mutations in the genes of Gs alpha subunit (Gs alpha), Gi2 alpha subunit (Gi2 alpha), H-ras, K-ras, and N-ras by a polymerase chain reaction-direct sequencing method. An adenomatous goiter and a follicular adenoma showed double mutations at codon 227 and 231, and 4 papillary carcinomas showed mutation at codon 231 of the Gs alpha gene. An adenomatous goiter, a follicular adenoma, and a papillary carcinoma showed a missense mutation in codon 13 of the K-ras gene. There were no such missense mutations of these G-protein or ras genes in medullary carcinomas. These data indicate that the genetic events involved in the oncogenesis of parafollicular C-cells are different from those of thyroid follicular cells, in which missense mutations of Gs alpha and ras genes seem to play important roles in tumorigenesis.
我们采用聚合酶链反应-直接测序法，研究了43例甲状腺肿瘤，包括5例腺瘤性甲状腺肿、7例滤泡性腺瘤、22例乳头状癌和9例髓样癌，检测Gsα亚基（Gsα）、Gi2α亚基（Gi2α）、H-ras、K-ras和N-ras基因中的点突变情况。1例腺瘤性甲状腺肿和1例滤泡性腺瘤在第227和231密码子处出现双重突变，4例乳头状癌在Gsα基因的第231密码子处出现突变。1例腺瘤性甲状腺肿、1例滤泡性腺瘤和1例乳头状癌在K-ras基因的第13密码子处出现错义突变。髓样癌中未出现这些G蛋白或ras基因的错义突变。这些数据表明，甲状腺滤泡旁C细胞肿瘤发生过程中的遗传事件与甲状腺滤泡细胞不同，在甲状腺滤泡细胞中，Gsα和ras基因的错义突变似乎在肿瘤发生中起重要作用。
