Soeffing W J, Timms B G
Department of Anatomy and Structural Biology, University of South Dakota School of Medicine, Vermillion 57069, USA.
J Androl. 1995 May-Jun;16(3):197-208.
Immunocytochemical techniques labeled androgen receptor and cell-specific cytokeratins in the basal cells of rat ventral prostate. In addition, nonradioactive in situ hybridization verified the production of androgen receptor transcripts in the basal cells. Androgen receptors and transcripts were localized in the nuclei and cytoplasm of the adult basal cells using these two techniques. Monoclonal anti-cytokeratin antibodies identified temporal changes in the expression of basal cell-specific intermediate filaments of fetal, neonatal, normal adult, orchidectomized adult, and testosterone-treated orchidectomized adult prostates. Labeling intensity of the basal cells was elevated during development when compared to the staining in normal adult tissue. Orchidectomized adults exhibited the greatest intensity of labeling, which decreased after testosterone treatment. The detection of androgen receptor and its transcripts in the basal cells supports the hypothesis that these cells are androgen responsive. The observed change in the anti-cytokeratin staining patterns of these cells during development, growth, and regression is an indirect measure of androgenic influence. The androgen-repressed cytokeratin expression in basal cells is similar to that found in prostatic luminal cells.
免疫细胞化学技术标记了大鼠腹侧前列腺基底细胞中的雄激素受体和细胞特异性细胞角蛋白。此外，非放射性原位杂交证实了基底细胞中雄激素受体转录本的产生。使用这两种技术，雄激素受体和转录本定位于成年基底细胞的细胞核和细胞质中。单克隆抗细胞角蛋白抗体鉴定了胎儿、新生儿、正常成年、去势成年和睾酮处理的去势成年前列腺基底细胞特异性中间丝表达的时间变化。与正常成年组织中的染色相比，发育过程中基底细胞的标记强度升高。去势成年大鼠的标记强度最大，睾酮处理后降低。在基底细胞中检测到雄激素受体及其转录本支持了这些细胞对雄激素有反应的假说。在发育、生长和消退过程中观察到的这些细胞抗细胞角蛋白染色模式的变化是雄激素影响的间接指标。基底细胞中雄激素抑制的细胞角蛋白表达与前列腺管腔细胞中的相似。
