Charland N, Kellens J T, Caya F, Gottschalk M
Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montréal, St.-Hyacinthe, Canada.
J Clin Microbiol. 1995 Aug;33(8):2220-1. doi: 10.1128/jcm.33.8.2220-2221.1995.
The 35 Streptococcus suis capsular-type reference strains as well as 45 field strains of type 2 were tested with sialic acid-binding lectins from Sambucus nigra (SNA I), Triticum vulgaris, Maackia amurensis, Homarus americanus, and Limax flavus. Only types 1, 1/2, 2, 14, 15, and 16 agglutinated with SNA I and/or the T. vulgaris lectin. All field strains agglutinated only with SNA I. Reaction with SNA I was probably due to the sialic acid moiety since it disappeared after sialidase treatment. These results confirm the presence of sialic acid in S. suis with the possible terminal sequence N-acetylneuraminic acid-alpha(2,6)GalNAc.
用来自黑接骨木(SNA I)、普通小麦、山槐、美洲螯龙虾和黄蛞蝓的唾液酸结合凝集素对35株猪链球菌荚膜型参考菌株以及45株2型田间菌株进行了检测。只有1型、1/2型、2型、14型、15型和16型与SNA I和/或普通小麦凝集素发生凝集。所有田间菌株仅与SNA I发生凝集。与SNA I的反应可能归因于唾液酸部分,因为经唾液酸酶处理后反应消失。这些结果证实了猪链球菌中存在唾液酸,其可能的末端序列为N-乙酰神经氨酸-α(2,6)GalNAc。