Ammermüller J, Kolb H
Department of Neurobiology, University of Oldenburg, Germany.
J Comp Neurol. 1995 Jul 17;358(1):1-34. doi: 10.1002/cne.903580102.
Intracellular recordings and dye injections of Lucifer yellow, horseradish peroxidase, or Neurobiotin were made in bipolar, amacrine, and ganglion cells of the Pseudemys turtle retina. By using a standard light-stimulation protocol in a sample of 375 labeled neurons, we were able to identify morphological and physiological characteristics of 11 types of bipolar cell, 37 types of amacrine cell, and 24 types of ganglion cell. To make sense of these data, we have chosen to group the 72 essentially different neuron types into traditional, functionally significant pathways. In this paper we look at the neuronal types in the inner plexiform layer (IPL) in terms of their contribution to generalized luminosity responses such as sustained ON- or OFF-center and transient ON-OFF ganglion cells; in the companion paper (J. Ammermüller, J.F. Muller, and H. Kolb, 1995, J. Comp. Neurol. 358:35-62) we look at them in terms of their involvement in color opponency and directional selectivity. A functional organization of the turtle IPL into OFF sublaminae (strata 1 and 2) and ON sublaminae (strata 3, 4, and 5), as has been described for other vertebrate retinas, was quite clear for two varieties of OFF-center bipolar cells (B4 and B5) and for all four types of sustained ON-center bipolar cell (B1, B2, B6, and B7). Thus, we found no sustained ON-center bipolar cell terminating in strata 1 and 2. We did, however, see three varieties of sustained OFF-center bipolar cells (B3, B9, and B10) having axon terminals in strata 3-5 (the ON sublamina) in addition to their terminations in stratum 1 or 2 (the OFF sublamina). Monostratified sustained ON- and OFF-center amacrine and ganglion cells rigidly obeyed the border of ON and OFF sublaminae. However, multistratified and diffuse sustained amacrine and ganglion cells could be either ON-center or OFF-center, and they did not strictly obey the border: such ON-center cells always had processes in one of the ON sublaminae (strata 3-5), and the equivalent OFF-center cells always had processes in one of the OFF sublaminae (strata 1 and 2). Monostratified transient amacrine and ganglion cells were concentrated in the middle of the IPL (around stratum 3), whereas bi-, tri-, or multistratified transient amacrine or ganglion cells always had processes in both the ON and the OFF sublaminae.(ABSTRACT TRUNCATED AT 400 WORDS)
我们对伪龟视网膜的双极细胞、无长突细胞和神经节细胞进行了细胞内记录，并注射了路西法黄、辣根过氧化物酶或神经生物素。通过对375个标记神经元样本使用标准光刺激方案，我们能够识别出11种双极细胞、37种无长突细胞和24种神经节细胞的形态和生理特征。为了理解这些数据，我们选择将这72种本质上不同的神经元类型归为传统的、功能上有意义的通路。在本文中，我们从它们对诸如持续的ON或OFF中心以及瞬态ON - OFF神经节细胞等广义亮度反应的贡献方面来研究内网状层（IPL）中的神经元类型；在配套论文（J. Ammermüller、J.F. Muller和H. Kolb，1995年，《比较神经学杂志》358:35 - 62）中，我们从它们参与颜色拮抗和方向选择性方面来研究它们。正如在其他脊椎动物视网膜中所描述的那样，龟IPL的功能组织分为OFF亚层（第1和第2层）和ON亚层（第3、4和第5层），对于两种OFF中心双极细胞（B4和B5）以及所有四种持续的ON中心双极细胞（B1、B2、B6和B7）来说非常清晰。因此，我们没有发现持续的ON中心双极细胞终止于第1和第2层。然而，我们确实看到三种持续的OFF中心双极细胞（B3、B9和B10），除了它们在第1或第2层（OFF亚层）的终末外，其轴突终末还位于第3 - 5层（ON亚层）。单分层的持续ON和OFF中心无长突细胞和神经节细胞严格遵循ON和OFF亚层的边界。然而，多分层和弥散的持续无长突细胞和神经节细胞可以是ON中心或OFF中心，并且它们并不严格遵循边界：这样的ON中心细胞总是在其中一个ON亚层（第3 - 5层）中有突起，而等效的OFF中心细胞总是在其中一个OFF亚层（第1和第2层）中有突起。单分层的瞬态无长突细胞和神经节细胞集中在IPL中部（第3层附近），而双分层、三分层或多分层的瞬态无长突细胞或神经节细胞总是在ON和OFF亚层中都有突起。（摘要截于400字）
