Byrd C A, Brunjes P C
Department of Psychology, University of Virginia, Charlottesville 22903, USA.
J Comp Neurol. 1995 Jul 24;358(2):247-59. doi: 10.1002/cne.903580207.
The zebrafish, Danio rerio, is becoming an important model system for developmental studies. We have used a variety of histological techniques to characterize the adult structure of the olfactory system in this teleost to form a base for future developmental work. The olfactory epithelium in this fish contains ciliated and microvillar sensory neurons, microvillar supporting cells, secretory goblet cells, and basal cells, and the adjacent nonsensory epithelium contains ciliated supporting cells. The olfactory bulb is a diffusely organized structure with four laminae: olfactory nerve, glomerular, mixed mitral cell/plexiform, and granule cell layers. These structures and the synapses observed in the olfactory bulb are typical of what is found in other vertebrates. We also examined the distribution of several neurotransmitter markers (tyrosine hydroxylase, neuropeptide Y, dopamine-beta-hydroxylase, and serotonin) in the olfactory bulb. Antibodies to neuropeptide Y, dopamine-beta-hydroxylase, and serotonin labeled fibers in the olfactory bulb and cell bodies in caudal regions of the brain in distributions comparable to other species. Tyrosine hydroxylase immunoreactivity was observed in a set of intrinsic bulb neurons with extensive processes in the glomerular layer. In addition, the structural proteins glial fibrillary acidic protein and vimentin have distributions similar to those in the olfactory bulbs of other animals. Thus, the adult olfactory structures are analogous to the structures in other vertebrate animals in morphology and chemical neuroanatomy. This similarity, along with its numerous advantages for developmental studies, makes the zebrafish a good model for studies of olfaction and forebrain maturation.
斑马鱼（Danio rerio）正成为发育研究的重要模型系统。我们运用了多种组织学技术来描绘这种硬骨鱼成年嗅觉系统的结构，为未来的发育研究奠定基础。这种鱼的嗅觉上皮包含纤毛和微绒毛感觉神经元、微绒毛支持细胞、分泌杯状细胞和基底细胞，相邻的非感觉上皮含有纤毛支持细胞。嗅球是一个结构分散的组织，有四层：嗅神经层、肾小球层、混合的二尖瓣细胞/丛状层和颗粒细胞层。这些结构以及在嗅球中观察到的突触是其他脊椎动物中常见的典型结构。我们还研究了几种神经递质标志物（酪氨酸羟化酶、神经肽Y、多巴胺-β-羟化酶和5-羟色胺）在嗅球中的分布。针对神经肽Y、多巴胺-β-羟化酶和5-羟色胺的抗体标记了嗅球中的纤维以及大脑尾部区域的细胞体，其分布与其他物种相当。在一组内在的嗅球神经元中观察到酪氨酸羟化酶免疫反应性，这些神经元在肾小球层有广泛的突起。此外，结构蛋白胶质纤维酸性蛋白和波形蛋白的分布与其他动物嗅球中的分布相似。因此，成年嗅觉结构在形态学和化学神经解剖学上与其他脊椎动物的结构相似。这种相似性，连同其在发育研究中的众多优势，使斑马鱼成为嗅觉和前脑成熟研究的良好模型。
