Virtanen T, Maggi E, Manetti R, Piccinni M P, Sampognaro S, Parronchi P, De Carli M, Zuccati G, Romognani S
Department of Clinical Microbiology, University of Kuopio, Finland.
J Allergy Clin Immunol. 1995 Sep;96(3):411-20. doi: 10.1016/s0091-6749(95)70061-7.
More than 500 CD4+ T-cell clones (TCCs) derived from the skin of eight patients with atopic dermatitis (AD), two patients with nonatopic dermatologic disorders, two patients with allergic rhinitis, and one healthy nonatopic donor were analyzed for both their pattern of cytokine production and their antigen specificity. The proportions of TCCs from patients with AD producing interleukin-4 in response to stimulation with phorbol 12-myristate 13-acetate plus anti-CD3 antibody were higher, whereas the proportions of interferon-gamma--producing TCCs were lower than those of control subjects. In two patients with AD, the majority of TCCs had a TH2/TH0-like phenotype, whereas in six patients with AD a TH1/TH0-like phenotype was prevalent. TCCs with a TH2/TH0-like phenotype were also isolated from the healthy skin of two patients with allergic rhinitis and one nonatopic donor. In contrast, no TH2-like TCCs were derived from the skin of the two patients with dermatologic disorders of nonallergic origin. No unambiguous correlations was found between the proportions of TCCs producing interleukin-4 or interferon-gamma (or of TCCs with TH2- or TH1-like profile) and the level of total serum IgE, suggesting that CD4+ T cells infiltrating the atopic skin do not play a major role in the production of serum IgE antibodies. When TCCs from five patients with AD were examined for their specificity, the proportions of allergen-specific (Dermatophagoides pteronyssinus and Lol p 1) clones were consistently 6% or lower even in patients with high titers of ryegrassor D. pteronyssinus-specific IgE antibodies. Because similar percentages of allergen-specific TCCs were found in skin from two healthy control subjects, the role of aeroallergens in favoring and maintaining skin lesions in patients with AD remains unclear.
对来自8例特应性皮炎（AD）患者、2例非特应性皮肤病患者、2例过敏性鼻炎患者以及1例健康非特应供体皮肤的500多个CD4 + T细胞克隆（TCC）进行了细胞因子产生模式和抗原特异性分析。与用佛波醇12 -肉豆蔻酸酯13 -乙酸酯加抗CD3抗体刺激后产生白细胞介素-4的AD患者TCC比例相比，对照组更高，而产生干扰素-γ的TCC比例更低。在2例AD患者中，大多数TCC具有TH2 / TH0样表型，而在6例AD患者中，TH1 / TH0样表型占主导。具有TH2 / TH0样表型的TCC也从2例过敏性鼻炎患者和1例非特应供体的健康皮肤中分离得到。相比之下，2例非过敏性皮肤病患者的皮肤中未分离出TH2样TCC。未发现产生白细胞介素-4或干扰素-γ的TCC比例（或具有TH2或TH1样特征的TCC比例）与血清总IgE水平之间存在明确的相关性，这表明浸润特应性皮肤的CD4 + T细胞在血清IgE抗体产生中不起主要作用。当检测5例AD患者的TCC特异性时，即使在黑麦草或粉尘螨特异性IgE抗体滴度高的患者中，过敏原特异性（粉尘螨和黑麦草Lol p 1）克隆的比例始终为6%或更低。由于在2例健康对照受试者的皮肤中发现了相似百分比的过敏原特异性TCC，气传过敏原在AD患者中促进和维持皮肤病变中的作用仍不清楚。
