Wilson G, Ren H, Laurent J
School of Optometry, University of Alabama at Birmingham 35294-4390, USA.
J Am Optom Assoc. 1995 Jul;66(7):435-41.
There is some difference of opinion in the literature about the nature of fluorescein staining of the epithelial surface. Most authors support the view that fluorescein staining is due to drop out of cells and pooling of fluorescein in the footprint. Others believe that fluorescein fills intercellular spaces. Others suggest that cells themselves stain with fluorescein.
Rabbit corneas were stained with fluorescein and examined with the biomicroscope and later with a higher magnification epifluorescent microscope following excision.
Fluorescein staining was shown to be due to staining of individual cells. No evidence was found to support the contention that fluorescein resides in areas of cell drop out, or that staining was due to filling of intercellular spaces. Micropunctate staining is shown to be due to staining of cells in an optimum manner, which is referred to as hyperfluorescence.
The recognition that fluorescein stains cells, even in the case of epithelial abrasion, allows reinterpretation of many staining phenomena, including "salt and pepper" staining.
关于上皮表面荧光素染色的本质，文献中存在一些不同观点。大多数作者支持这样的观点，即荧光素染色是由于细胞脱落以及荧光素在细胞脱落后留下的痕迹中积聚所致。另一些人认为荧光素填充细胞间隙。还有一些人提出细胞自身被荧光素染色。
用荧光素对兔角膜进行染色，先用生物显微镜检查，切除后再用高倍荧光显微镜检查。
荧光素染色显示是由于单个细胞被染色。没有证据支持荧光素存在于细胞脱落区域或染色是由于细胞间隙被填充的观点。微点状染色显示是由于细胞以最佳方式被染色，这被称为高荧光。
认识到即使在上皮擦伤的情况下荧光素也能对细胞进行染色，这使得对许多染色现象，包括“椒盐”染色，有了重新的解释。
