Troisi Mario, Caruso Ciro, D'Andrea Luca, Rinaldi Michele, Piscopo Raffaele, Troisi Salvatore, Costagliola Ciro
Eye Clinic, Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via Pansini n. 5, 80131 Naples, Italy.
Corneal Transplant Center, Pellegrini Hospital, Via Portamedina alla Pignasecca, 41, 80127 Napoli, Italy.
Life (Basel). 2024 May 21;14(6):653. doi: 10.3390/life14060653.
Ocular surface staining for assessing corneal and conjunctival epithelium integrity is typically conducted using fluorescein, lissamine green, or rose Bengal dyes. Recently, a novel vital dye, REmark, based on riboflavin, has been proposed for ocular surface examination. In the management of corneal and ocular surface diseases (OSD), the use of contact lenses is integral to therapeutic strategies. This study explores the compatibility of REmark with four different types of disposable or bi-weekly soft contact lenses. Morphological variations observed under stereomicroscopy and ultraviolet (UV) ray transmittance in the visible spectrum (VIS) were evaluated at 2 and 4 h post-immersion of the contact lenses in both the original fluid and the new dye. The findings indicate no significant differences between the group treated with the original liquid and those immersed in REmark, except for a yellow hue observed in the latter group, which dissipates after 8 h in physiological solution. This study highlights the potential of utilizing the new vital dye for ophthalmologic examinations even in the presence of applied soft contact lenses, offering a promising avenue for improved diagnostic practices and patient comfort.
用于评估角膜和结膜上皮完整性的眼表染色通常使用荧光素、丽丝胺绿或孟加拉玫瑰红染料进行。最近，一种基于核黄素的新型活性染料REmark已被提议用于眼表检查。在角膜和眼表疾病（OSD）的治疗中，隐形眼镜的使用是治疗策略的重要组成部分。本研究探讨了REmark与四种不同类型的一次性或两周更换一次的软性隐形眼镜的兼容性。在将隐形眼镜分别浸泡在原液和新染料中2小时和4小时后，评估了在立体显微镜下观察到的形态变化以及可见光谱（VIS）中的紫外线（UV）透过率。研究结果表明，除了后一组观察到的黄色色调在生理溶液中8小时后消散外，用原液处理的组和浸泡在REmark中的组之间没有显著差异。这项研究突出了即使在佩戴软性隐形眼镜的情况下使用这种新型活性染料进行眼科检查的潜力，为改进诊断方法和提高患者舒适度提供了一条有前景的途径。
