Atoji Y, Hirasawa Y, Yamamoto Y, Suzuki Y
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Japan.
J Auton Nerv Syst. 1995 Jun 25;53(2-3):185-94. doi: 10.1016/0165-1838(94)00172-g.
The origin of the neurotensin-containing nerve fibers in the digestive tract of the chicken has been investigated with the use of colchicine and immunohistochemistry. Neurotensin-immunoreactive nerve fibers were found in the smooth muscle layers from the esophagus to the duodenum. Their density of distribution was very high in the esophagus and crop (maximum mean value: 1315/mm2 of sectional area in the lamina muscularis mucosae of the crop) and decreased progressively to the duodenum. Neurotensin-immunoreactive neuronal cell bodies were observed after colchicine treatment in the submucosal plexuses of the esophagus and crop and in the myenteric plexuses of the esophagus, crop, proventriculus and gizzard, and they extended varicose fibers. The number of neurotensin-immunoreactive cell bodies was high in the myenteric plexus of the gizzard (28.3 +/- 2.7/ganglion) but low in the plexuses of the esophagus, crop and proventriculus. Seven days after cutting the glossopharyngeal nerve and vagus nerve unilaterally, the number and extent of neurotensin-immunostained structures in the smooth muscle layers from the esophagus to the gizzard did not show any significant difference between operated and unoperated sides. These results indicate that in the chicken the great majority of neurotensin-immunoreactive enteric fibers originate in the intramural plexuses of the upper digestive wall and are mainly distributed to smooth muscle cells.
利用秋水仙碱和免疫组织化学方法，对鸡消化道中含神经降压素的神经纤维的起源进行了研究。在从食管到十二指肠的平滑肌层中发现了神经降压素免疫反应性神经纤维。它们在食管和嗉囊中的分布密度非常高（最大值均值：嗉囊黏膜肌层横截面积为1315/mm²），并向十二指肠逐渐降低。秋水仙碱处理后，在食管和嗉囊的黏膜下神经丛以及食管、嗉囊、腺胃和肌胃的肌间神经丛中观察到神经降压素免疫反应性神经元细胞体，它们延伸出曲张纤维。肌胃肌间神经丛中神经降压素免疫反应性细胞体的数量较多（28.3±2.7/神经节），而在食管、嗉囊和腺胃的神经丛中数量较少。单侧切断舌咽神经和迷走神经7天后，从食管到肌胃的平滑肌层中神经降压素免疫染色结构的数量和范围在手术侧和未手术侧之间没有显示出任何显著差异。这些结果表明，在鸡中，绝大多数神经降压素免疫反应性肠纤维起源于上消化道壁的壁内神经丛，并主要分布于平滑肌细胞。
