表面免疫球蛋白M诱导B细胞凋亡中磷脂酶C-γ2激活的需求
Requirement of phospholipase C-gamma 2 activation in surface immunoglobulin M-induced B cell apoptosis.
作者信息
Takata M, Homma Y, Kurosaki T
机构信息
Department of Cardiovascular Molecular Biology, Lederle Laboratories, Pearl River, New York 10965, USA.
出版信息
J Exp Med. 1995 Oct 1;182(4):907-14. doi: 10.1084/jem.182.4.907.
Abstract
Surface IgM (sIgM) stimulation induces the tyrosine phosphorylation of multiple cellular substrates, including phospholipase C (PLC)-gamma 2, which is involved in the activation of phosphatidylinositol pathway. DT40 B cells underwent apoptotic cell death when activated through sIgM, a phenomenon that is related to elimination of self-reactive B cells. To examine the roles of PLC-gamma 2 in sIgM signaling, we have generated DT40 cells deficient in PLC-gamma 2 Cross-linking of sIgM on PLC-gamma 2-deficient cells evoked neither inositol 1,4,5-trisphosphate nor calcium mobilization. In PLC-gamma 2- or Syk-deficient DT40 cells, the induction of apoptosis was blocked, but was still observed in Lyn-deficient cells. Src homology 2 domains of PLC-gamma 2 were essential for both its activation and sIgM-induced apoptosis. Since tyrosine phosphorylation of PLC-gamma 2 is mediated by Syk, these results indicate that activation of PLC-gamma 2 through Syk is required for sIgM-induced apoptosis.
摘要
表面免疫球蛋白M（sIgM）刺激可诱导多种细胞底物的酪氨酸磷酸化，包括参与磷脂酰肌醇途径激活的磷脂酶C（PLC）-γ2。当通过sIgM激活时，DT40 B细胞会经历凋亡性细胞死亡，这一现象与自身反应性B细胞的清除有关。为了研究PLC-γ2在sIgM信号传导中的作用，我们构建了PLC-γ2缺陷的DT40细胞。在PLC-γ2缺陷细胞上对sIgM进行交联既不会引起肌醇1,4,5-三磷酸的产生，也不会引起钙动员。在PLC-γ2或Syk缺陷的DT40细胞中，凋亡的诱导被阻断，但在Lyn缺陷细胞中仍可观察到凋亡。PLC-γ2的Src同源2结构域对其激活和sIgM诱导的凋亡都至关重要。由于PLC-γ2的酪氨酸磷酸化是由Syk介导的，这些结果表明通过Syk激活PLC-γ2是sIgM诱导凋亡所必需的。
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本文引用的文献
1
B-cell antigen receptor motifs have redundant signalling capabilities and bind the tyrosine kinases PTK72, Lyn and Fyn.B细胞抗原受体基序具有冗余的信号传导能力，并与酪氨酸激酶PTK72、Lyn和Fyn结合。
Curr Biol. 1993 Oct 1;3(10):645-57. doi: 10.1016/0960-9822(93)90062-s.
2
Association with B-cell-antigen receptor with protein-tyrosine kinase p72syk and activation by engagement of membrane IgM.B细胞抗原受体与蛋白酪氨酸激酶p72syk的关联以及通过膜IgM的结合而激活。
Eur J Biochem. 1993 Apr 1;213(1):455-9. doi: 10.1111/j.1432-1033.1993.tb17781.x.
3
Programmed cell death by bcl-2-dependent and independent mechanisms in B lymphoma cells.B淋巴瘤细胞中通过bcl-2依赖和非依赖机制的程序性细胞死亡
EMBO J. 1993 Apr;12(4):1555-60. doi: 10.1002/j.1460-2075.1993.tb05799.x.
4
Abnormal transcription factor induction through the surface immunoglobulin M receptor of B-1 lymphocytes.通过B-1淋巴细胞的表面免疫球蛋白M受体诱导异常转录因子
J Exp Med. 1993 Mar 1;177(3):857-61. doi: 10.1084/jem.177.3.857.
5
Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair.Bcl-2基因缺陷型小鼠表现出暴发性淋巴细胞凋亡、多囊肾和毛发色素减退。
Cell. 1993 Oct 22;75(2):229-40. doi: 10.1016/0092-8674(93)80065-m.
6
The signal for capacitative calcium entry.钙库调控性钙内流信号。
Cell. 1993 Oct 22;75(2):199-201. doi: 10.1016/0092-8674(93)80061-i.
7
Signalling function of the B-cell antigen receptors.B细胞抗原受体的信号传导功能。
Immunol Rev. 1993 Apr;132:125-46. doi: 10.1111/j.1600-065x.1993.tb00840.x.
8
Inositol trisphosphate and calcium signalling.肌醇三磷酸与钙信号传导
Nature. 1993 Jan 28;361(6410):315-25. doi: 10.1038/361315a0.
9
Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice.Bcl-2纯合突变嵌合小鼠中淋巴系统的消失。
Science. 1993 Sep 17;261(5128):1584-8. doi: 10.1126/science.8372353.
10
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.Bcl-2在体内与一个保守的同源物Bax形成异二聚体，后者会加速程序性细胞死亡。
Cell. 1993 Aug 27;74(4):609-19. doi: 10.1016/0092-8674(93)90509-o.
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