Lundgren A L, Zimmermann W, Bode L, Czech G, Gosztonyi G, Lindberg R, Ludwig H
Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
J Gen Virol. 1995 Sep;76 ( Pt 9):2215-22. doi: 10.1099/0022-1317-76-9-2215.
A Borna disease virus (BDV)-like agent was isolated from the central nervous system (CNS) of cats with a spontaneous non-suppurative encephalomyelitis ('staggering disease'). In contrast to the rabbit-adapted BDV strain V, which can be propagated in several primary and permanent cell cultures, the cat virus grew only in embryonic mink brain cells. Infection of adult Wistar rats with feline brain tissue material did not result in clinical disease during a period of 5 months, nor in growth of infectious virus in the brain. However, using the brain suspension of a newborn rat inoculated with feline brain tissue material, it was possible to induce typical Borna disease (BD) in four adult rats. This indicates a possible adaptation of the cat virus during passages in rats. By the use of an RT-PCR technique, BDV-specific RNA could be detected in a majority of brain samples from diseased cats. BDV-specific antigen was demonstrated in feline CNS samples both by immunohistochemistry and ELISA. However, the amount of BDV RNA and BDV antigen was less in the cats as compared to horses with BD, providing further support for the notion that a distinct feline BDV strain exists.
从患有自发性非化脓性脑脊髓炎（“蹒跚病”）的猫的中枢神经系统（CNS）中分离出一种类博尔纳病病毒（BDV）病原体。与可在多种原代和永生细胞培养物中增殖的兔适应BDV株V不同，猫病毒仅在胚胎水貂脑细胞中生长。用猫脑组织材料感染成年Wistar大鼠，在5个月期间未引发临床疾病，大脑中也未出现传染性病毒生长。然而，使用接种了猫脑组织材料的新生大鼠的脑悬液，有可能在四只成年大鼠中诱发典型的博尔纳病（BD）。这表明猫病毒在大鼠传代过程中可能发生了适应性变化。通过使用RT-PCR技术，在大多数患病猫的脑样本中可检测到BDV特异性RNA。通过免疫组织化学和ELISA在猫CNS样本中证实了BDV特异性抗原。然而，与患BD的马相比，猫体内的BDV RNA和BDV抗原量较少，这进一步支持了存在一种独特的猫BDV株的观点。
