Bastianelli E, Pochet R
Laboratoire d'Anatomie-Pathologique, Hopital Erasme, Bruxelles, Belgium.
J Pineal Res. 1995 Apr;18(3):127-34. doi: 10.1111/j.1600-079x.1995.tb00150.x.
Profound morphological modifications occur during postnatal development of the rat pineal gland. We have immunohistochemically followed those events from postnatal day 1 to 20 by using three cytoarchitectonic markers (S-100, calbindin-D28k, and calretinin) that belong to the calmodulin/troponin C calcium-binding protein family. In the developing rat pineal, anticalbindin-D28k antibody labels three cell types: immature and mature astrocytes and perivascular type II pinealocytes. During development, calbindin-D28k positive cells migrate from the base of the pineal stalk into the superficial part of the pineal. Calbindin-D28k, usually used as a neuronal marker in the central nervous system, recognizes in rat pineal precursor astrocytes 5 days before S-100 and labels a subpopulation somewhat different from S-100 positive astrocytes. Calretinin immunoreactivity appeared in the postero-superior part of the pineal and was abundant until postnatal day 5, then its density dramatically felt to leave, after postnatal day 20, an occasional population of cells whose morphology is compatible with neuron-like cells.
大鼠松果体在出生后发育过程中会发生深刻的形态学改变。我们使用属于钙调蛋白/肌钙蛋白C钙结合蛋白家族的三种细胞结构标记物（S-100、钙结合蛋白-D28k和钙视网膜蛋白），通过免疫组织化学方法追踪了从出生后第1天到第20天的这些事件。在发育中的大鼠松果体中，抗钙结合蛋白-D28k抗体标记三种细胞类型：未成熟和成熟的星形胶质细胞以及血管周围II型松果体细胞。在发育过程中，钙结合蛋白-D28k阳性细胞从松果体柄基部迁移到松果体的表层。钙结合蛋白-D28k通常在中枢神经系统中用作神经元标记物，在大鼠松果体前体星形胶质细胞中比S-100早5天被识别，并且标记的亚群与S-100阳性星形胶质细胞略有不同。钙视网膜蛋白免疫反应出现在松果体的后上部，在出生后第5天之前丰富，然后其密度急剧下降，在出生后第20天之后，留下偶尔一群形态与神经元样细胞相符的细胞。
