外切酶S和蛋白酶对小鼠内源性铜绿假单胞菌菌血症的影响。

The influence of exo-enzyme S and proteases on endogenous Pseudomonas aeruginosa bacteraemia in mice.

作者信息

Hirakata Y, Furuya N, Tateda K, Matsumoto T, Yamaguchi K

机构信息

Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.

出版信息

J Med Microbiol. 1995 Oct;43(4):258-61. doi: 10.1099/00222615-43-4-258.

DOI:10.1099/00222615-43-4-258

PMID:7562986

Abstract

The role of Pseudomonas aeruginosa exo-enzymes was evaluated in a murine model of endogenous bacteraemia in which the bacteria invaded the bloodstream after oral dosing. Although an elastase mutant PAO-E64 was as virulent as its parent strain PAO1, an exo-enzyme S-deficient mutant, DG1-ExS5 and alkaline protease mutants PAKS-16, PAKS-17, PAKS-19, were less virulent than their parent strains, DG1 and PAKS-1, respectively (p < 0.01). Thus exo-enzyme S and alkaline protease, but not elastase, appear to contribute to the pathogenicity of P. aeruginosa in this model.

摘要

在口服给药后细菌侵入血流的内源性菌血症小鼠模型中，对铜绿假单胞菌外酶的作用进行了评估。尽管弹性蛋白酶突变体PAO-E64与其亲本菌株PAO1的毒力相同，但外酶S缺陷突变体DG1-ExS5和碱性蛋白酶突变体PAKS-16、PAKS-17、PAKS-19的毒力分别低于其亲本菌株DG1和PAKS-1（p<0.01）。因此，在该模型中，外酶S和碱性蛋白酶而非弹性蛋白酶似乎对铜绿假单胞菌的致病性有影响。