Elsheikh L E, Kronevi T, Wretlind B, Abaas S, Iglewski B H
National Veterinary Institute, Uppsala, Sweden.
Vet Microbiol. 1987 Mar;13(3):281-9. doi: 10.1016/0378-1135(87)90090-3.
Anaesthetized mink were inoculated intratracheally with an elastase-producing Pseudomonas aeruginosa strain (PAO1) and two mutants derived from PAO1 with defective elastase formation (strains PAO1-E64 and PAO1-las-16). Survival times were prolonged in mink infected with the mutants, and microscopic examination of lungs showed that the elastase-positive wild type strain produced more pronounced tissue damage and haemorrhages than did the elastase-defective mutant strains. The strains PAO1 and PAO1-las-16 were also compared to three strains isolated from natural infection in mink which differed in elastase production. The mink strains with high or moderate elastase production produced more severe lung damage and were associated with a higher mortality than the other strains tested. The results indicate that P. aeruginosa may enhance the virulence of the bacterium in lung infections.
将麻醉后的水貂经气管内接种产弹性蛋白酶的铜绿假单胞菌菌株（PAO1）以及从PAO1衍生而来的两个弹性蛋白酶形成缺陷的突变株（菌株PAO1-E64和PAO1-las-16）。感染突变株的水貂存活时间延长，对肺部进行显微镜检查发现，与弹性蛋白酶缺陷的突变株相比，弹性蛋白酶阳性的野生型菌株造成的组织损伤和出血更为明显。还将菌株PAO1和PAO1-las-16与从自然感染水貂中分离出的三株在弹性蛋白酶产生方面存在差异的菌株进行了比较。弹性蛋白酶产生量高或中等的水貂菌株造成的肺部损伤更严重，且与比其他测试菌株更高的死亡率相关。结果表明，铜绿假单胞菌可能会增强该细菌在肺部感染中的毒力。
