Bowden R A, Cloeckaert A, Zygmunt M S, Dubray G
Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, Centre de Recherches de Tours, Nouzilly, France.
J Med Microbiol. 1995 Nov;43(5):344-7. doi: 10.1099/00222615-43-5-344.
Brucella ovis, a naturally virulent rough Brucella species, is the aetiological agent of ram epididymitis. The identification of protective antigens is necessary to obtain a safe, specific subcellular vaccine. Monoclonal antibodies (MAbs) directed at both brucella outer-membrane proteins (OMPs) and rough lipopolysaccharide (R-LPS) in a mouse protection test were used to identify potential targets for humoral immunity. Mixtures of MAbs directed at the 16.5-, 25-27-, 31-34- and 36-38-kDa OMPs conferred significant protection 7 days after challenge with reference strain B. ovis 63/290 compared with controls receiving either saline or an anti-brucella O-polysaccharide MAb. Furthermore, an anti-R-LPS MAb tested alone conferred protection at a level comparable with that obtained with the mixture of anti-OMP MAbs. The combination of protective OMP MAbs with the anti-R-LPS MAb was also strongly protective. One combination of OMP MAbs, which bound intensely to B. ovis in vitro, was ineffective. These results indicate that B. ovis OMPs and R-LPS are targets for protective antibodies and that they can be regarded as candidates for ram epididymitis subcellular vaccines.
绵羊布鲁氏菌是一种天然具有毒力的粗糙型布鲁氏菌，是公羊附睾炎的病原体。鉴定保护性抗原对于获得安全、特异的亚细胞疫苗是必要的。在小鼠保护试验中，针对布鲁氏菌外膜蛋白（OMPs）和粗糙脂多糖（R-LPS）的单克隆抗体（MAbs）被用于识别体液免疫的潜在靶点。与接受生理盐水或抗布鲁氏菌O-多糖单克隆抗体的对照组相比，针对16.5 kDa、25 - 27 kDa、31 - 34 kDa和36 - 38 kDa OMPs的单克隆抗体混合物在受到绵羊布鲁氏菌参考菌株63/290攻击7天后提供了显著的保护作用。此外，单独测试的抗R-LPS单克隆抗体提供的保护水平与抗OMP单克隆抗体混合物相当。保护性OMP单克隆抗体与抗R-LPS单克隆抗体的组合也具有很强的保护作用。一种在体外与绵羊布鲁氏菌强烈结合的OMP单克隆抗体组合无效。这些结果表明，绵羊布鲁氏菌OMPs和R-LPS是保护性抗体的靶点，它们可被视为公羊附睾炎亚细胞疫苗的候选物。
