Bahk J Y, Hyun J S, Chung S H, Lee H, Kim M O, Lee B H, Choi W S
Department of Urology, Medical College, Gyeongsang National University, Chinju, Korea.
J Urol. 1995 Nov;154(5):1958-61.
To identify the expression of gonadotropin-releasing hormone (GnRH) messenger ribonuclueic acid (mRNA) and localize GnRH receptors in mature rat and adult human testes.
In situ hybridization and enzymatic receptor binding localization were performed.
GnRH mRNA was expressed within the seminiferous tubules in both mature rat and adult human testis. In rats, expression of GnRH mRNA was identified in the Sertoli cells and spermatogenic cells of some seminiferous tubules, but the other tubules did not express any hybridization signal. In humans, expression of GnRH mRNA was identified only in some spermatogenic cells in some seminiferous tubules. The receptors for GnRH were localized to cells in the interstitial tissues of the testis, probably Leydig cells.
The authors believe that the mature rat and adult human seminiferous tubular cells produce GnRH at the same specific stage of the spermatogenic cycle and that GnRH produced within seminiferous tubules, including Sertoli cells and spermatogenic cells, reacts with neighboring GnRH receptors in interstitial cells, including Leydig cells. The GnRH produced from the Sertoli cells in seminiferous tubules would react with GnRH receptors in interstitial cells as a paracrine hormone.
鉴定促性腺激素释放激素（GnRH）信使核糖核酸（mRNA）的表达，并在成熟大鼠和成年人类睾丸中定位GnRH受体。
进行原位杂交和酶促受体结合定位。
GnRH mRNA在成熟大鼠和成年人类睾丸的生精小管中均有表达。在大鼠中，GnRH mRNA在一些生精小管的支持细胞和生精细胞中表达，但其他小管未表达任何杂交信号。在人类中，GnRH mRNA仅在一些生精小管的部分生精细胞中表达。GnRH受体定位于睾丸间质组织中的细胞，可能是睾丸间质细胞。
作者认为，成熟大鼠和成年人类生精小管细胞在生精周期的同一特定阶段产生GnRH，并且生精小管内产生的GnRH，包括支持细胞和生精细胞产生的GnRH，与间质细胞（包括睾丸间质细胞）中的邻近GnRH受体发生反应。生精小管中支持细胞产生的GnRH将作为旁分泌激素与间质细胞中的GnRH受体发生反应。
