van der Hem K G, Dräger A M, Odding J H, Huijgens P C
Department of Hematology, Free University Hospital, Amsterdam, The Netherlands.
Leuk Res. 1995 Sep;19(9):651-7. doi: 10.1016/0145-2126(95)00052-p.
We examined the effect of bryostatin-5 (bryo-5) with and without a combination of myeloid growth promoting factors on human acute myeloid leukemia (AML) cell growth, maturation, and primary plating efficiency. In vitro treatment of AML samples with bryo-5 induced a macrophage-like cell differentiation as evidenced by morphological changes, esterase staining, and cell surface expression of CD11a and CD18. AML cells exposed to growth factors doubled their cell numbers following culture, this increase being abrogated by co-exposure to bryo-5. An antiproliferative effect, as well as the antagonistic interaction of bryo-5 with growth factors, was confirmed in methylcellulose clonogenic assays. Together, these findings indicate that the compound bryo-5 exerts an anti-proliferative effect on AML cells and counteracts growth factor induced leukemic proliferation.
我们研究了苔藓抑素-5（bryo-5）单独或与促进髓系生长因子联合使用对人急性髓系白血病（AML）细胞生长、成熟及原代接种效率的影响。用bryo-5对AML样本进行体外处理可诱导巨噬细胞样细胞分化，形态学改变、酯酶染色以及CD11a和CD18的细胞表面表达均可证明这一点。暴露于生长因子的AML细胞在培养后细胞数量翻倍，但同时暴露于bryo-5可消除这种增加。甲基纤维素克隆形成试验证实了bryo-5的抗增殖作用以及它与生长因子的拮抗相互作用。总之，这些发现表明化合物bryo-5对AML细胞具有抗增殖作用，并可对抗生长因子诱导的白血病细胞增殖。
