Clevens R A, Esclamado R, Naficy S
Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical Center, Ann Arbor 48109-0312, USA.
Laryngoscope. 1995 Oct;105(10):1043-8. doi: 10.1288/00005537-199510000-00006.
This study quantitatively examines the effects of laryngotracheal stenting with and without mucosal grafting on chondrogenesis in a rabbit model. Vascularized auricular perichondrium was transferred to a cricothyroid membrane (CTM) defect in three groups of New Zealand white rabbits: group A, CTM defect alone; group B, CTM defect with endotracheal tube stent; and group C, CTM defect with a buccal mucosa graft (BMG) interposed between the stent and perichondrium. The rabbits were sacrificed at 7 weeks for histologic examination and determination of cartilage thickness by digitometry. Chondrogenesis was not significantly affected by stenting (n = 11; 0.719 mm +/- 0.203 mm) or by BMG (n = 12; 0.775 mm +/- 0.203 mm) as compared with controls (n = 14; 0.695 mm +/- 0.216 mm) (P value = not significant). Laryngotracheal stenting and BMG do not adversely influence chondrogenesis in airway reconstruction with vascularized perichondrium in the rabbit model. The application of these principles in human airway reconstruction is discussed.
本研究定量检测了在兔模型中，行与不行黏膜移植的喉气管支架置入术对软骨形成的影响。将带血管的耳廓软骨膜移植到三组新西兰白兔的环甲膜（CTM）缺损处：A组，单纯CTM缺损；B组，CTM缺损加气管内导管支架；C组，CTM缺损，在支架与软骨膜之间置入颊黏膜移植片（BMG）。7周时处死兔子，进行组织学检查，并通过数字测量法测定软骨厚度。与对照组（n = 14；0.695 mm ± 0.216 mm）相比，支架置入术（n = 11；0.719 mm ± 0.203 mm）或BMG（n = 12；0.775 mm ± 0.203 mm）对软骨形成的影响不显著（P值无统计学意义）。在兔模型中，采用带血管软骨膜进行气道重建时，喉气管支架置入术和BMG对软骨形成无不利影响。本文还讨论了这些原则在人类气道重建中的应用。
