Seitz P K, Zhang R W, Simmons D J, Cooper C W
Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555-1031, USA.
Miner Electrolyte Metab. 1995;21(1-3):180-3.
A C-terminal analog of parathyroid hormone-related peptide (PTHrP), PTHrP(107-139), was found to stimulate cAMP production in three osteoblast cell preparations. The effect was studied most extensively in ROS 17/2.8 cells. The effect was dose-related and comparable in magnitude to that produced by PTHrP(1-34), but potency was lower. The functional significance of the cAMP effect is unknown, but preliminary findings indicated that PTHrP(107-139) also inhibited osteopontin mRNA levels in ROS 17/2.8 cells treated with peptide for 48 h. The results suggest that the carboxy-terminal region of PTHrP may play a role in bone metabolism by influencing osteoblast activity.
甲状旁腺激素相关肽（PTHrP）的C末端类似物PTHrP(107 - 139)，被发现可刺激三种成骨细胞制剂中的环磷酸腺苷（cAMP）生成。该效应在ROS 17/2.8细胞中得到了最广泛的研究。此效应呈剂量相关性，其强度与PTHrP(1 - 34)产生的效应相当，但效力较低。cAMP效应的功能意义尚不清楚，但初步研究结果表明，在经该肽处理48小时的ROS 17/2.8细胞中，PTHrP(107 - 139)也会抑制骨桥蛋白的mRNA水平。结果表明，PTHrP的羧基末端区域可能通过影响成骨细胞活性在骨代谢中发挥作用。
