Van Maele-Fabry G, Gofflot F, Clotman F, Picard J J
Laboratory of Developmental Genetics, Catholic University of Louvain, Louvain-la-Neuve, Belgium.
Neurotoxicol Teratol. 1995 Jul-Aug;17(4):497-506. doi: 10.1016/0892-0362(95)00009-g.
An immunostaining technique using monoclonal antibodies to a neurofilament protein has allowed us to visualize defects in the development of cranial nerves and ganglia of 10 to 10.5 days mouse embryos following exposure to ethanol in whole embryo culture. Reference patterns for development of cranial nerves and ganglia of control mouse embryos explanted and examined when they had 25 to 34 pairs of somites were established. Additionally, control mouse embryos were grown in whole embryo culture for 48 h, with culture being initiated in embryos having 6 to 7 somite pairs. At the end of the culture period, only minor differences were observed between the control groups. An experimental group of embryos was cultured in the presence of increasing doses (1.6, 3.2, 4, and 4.8 g/l) of ethanol. Defects were observed in the development of the glossopharyngeal and vagus nerves. These abnormalities included absence of the dorsal root (superior ganglion) of IX, star-like shape of inferior ganglion IX, disorganization of the rootlets of nerve X and abnormal fibers between the two nerves and ganglia. These results suggest that the migration and patterning of neural crest cells derived from r6 and r7 may be particularly affected by ethanol. The results also demonstrate the usefulness of this approach in evaluating the susceptibility of the developing cranial nerves to toxicant exposure.
一种使用针对神经丝蛋白的单克隆抗体的免疫染色技术，使我们能够在全胚胎培养中观察到暴露于乙醇后10至10.5天龄小鼠胚胎的颅神经和神经节发育缺陷。建立了在具有25至34对体节时移出并检查的对照小鼠胚胎颅神经和神经节发育的参考模式。此外，对照小鼠胚胎在全胚胎培养中生长48小时，培养从具有6至7对体节的胚胎开始。在培养期结束时，对照组之间仅观察到微小差异。一组实验组胚胎在递增剂量（1.6、3.2、4和4.8 g/l）的乙醇存在下培养。观察到舌咽神经和迷走神经发育存在缺陷。这些异常包括舌咽神经背根（上神经节）缺失、舌咽神经下神经节呈星状、迷走神经根丝紊乱以及两条神经和神经节之间的纤维异常。这些结果表明，源自r6和r7的神经嵴细胞的迁移和模式形成可能特别受乙醇影响。结果还证明了这种方法在评估发育中的颅神经对毒物暴露的易感性方面的有用性。
