具有转录活性的U2基因对于12型腺病毒诱导17q21 - 22处的脆性位点是必要且充分的。
The transcriptionally competent U2 gene is necessary and sufficient for adenovirus type 12 induction of the fragile site at 17q21-22.
作者信息
Gargano S, Wang P, Rusanganwa E, Bacchetti S
机构信息
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
出版信息
Mol Cell Biol. 1995 Nov;15(11):6256-61. doi: 10.1128/MCB.15.11.6256.
Abstract
Adenovirus type 12 induces four fragile sites upon infection of human cells. The U2 locus, consisting of up to 20 tandem repeats of a 5.8-kbp monomer, maps at the most sensitive of these sites at 17q21-22. We have previously shown that an artificial U2 locus integrated into the human genome generates a new virus-induced fragile site. To determine which elements within the U2 monomer are responsible for fragility, we constructed loci consisting of tandem repeats of subfragments of the U2 monomer. With this approach, we demonstrate that a transcriptionally competent U2 gene is necessary and sufficient for virus-induced fragility and that no other element within the 5.8-kbp monomer contributes to this effect.
摘要
12型腺病毒感染人类细胞后会诱导产生四个脆性位点。U2位点由一个5.8千碱基对单体的多达20个串联重复序列组成，定位于这些位点中最敏感的17q21 - 22处。我们之前已经表明，整合到人类基因组中的人工U2位点会产生一个新的病毒诱导脆性位点。为了确定U2单体中的哪些元件导致了脆性，我们构建了由U2单体亚片段串联重复组成的位点。通过这种方法，我们证明了一个具有转录活性的U2基因对于病毒诱导的脆性是必要且充分的，并且5.8千碱基对单体中的其他元件对此效应没有贡献。
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