体内烷基化剂在DNA中引发的姐妹染色单体交换损伤（FLE-SCE）的转归

Fate of lesions that elicit sister chromatid exchanges (FLE-SCE) produced in DNA by alkylating agents in vivo.

作者信息

Morales-Ramírez P, Rodríguez-Reyes R, Vallarino-Kelly T

机构信息

Departamento de Radiobiología, Instituto Nacional de Investigaciones Nucleares, México, D.F. Mexico.

出版信息

Mutat Res. 1995 Aug;344(1-2):13-26. doi: 10.1016/0165-1218(95)90034-9.

DOI:10.1016/0165-1218(95)90034-9

PMID:7565888

Abstract

A study was made on the effect of ethyl methanesulfonate (EMS) or dimethylnitrosamine (DMN) on the frequency of SCE occurring in the first, in the second or at the same locus in both divisions. This was done with a previously reported in vivo protocol which allowed us to determine the fate of lesions that elicit SCE (FLE-SCE) in two cell divisions after mutagen treatment. The results showed that EMS-induced DNA lesions that cause SCE are persistent, and that some of them were produced in the second-division or were generated from SCE non-inducing lesions. We inferred this by the analysis of the response in cell populations. DMN seems to induce SCE mainly during the second division, but by inhibiting DNA synthesis as was interpreted from the cell frequency distribution with respect to the number of SCE in first and in second cell division.

摘要

研究了甲磺酸乙酯（EMS）或二甲基亚硝胺（DMN）对第一次分裂、第二次分裂或两次分裂中同一基因座处姐妹染色单体交换（SCE）频率的影响。这是按照先前报道的体内实验方案进行的，该方案使我们能够确定诱变处理后两个细胞分裂中引发SCE的损伤命运（FLE-SCE）。结果表明，EMS诱导的导致SCE的DNA损伤是持续性的，其中一些是在第二次分裂中产生的，或者是由非诱导SCE的损伤产生的。我们通过对细胞群体反应的分析推断出这一点。DMN似乎主要在第二次分裂期间诱导SCE，但从第一次和第二次细胞分裂中SCE数量的细胞频率分布来看，这是通过抑制DNA合成来实现的。