Manjunath N, Correa M, Ardman M, Ardman B
Department of Medicine, New England Medical Center Hospitals, Boston, Massachusetts 02111, USA.
Nature. 1995 Oct 12;377(6549):535-8. doi: 10.1038/377535a0.
CD43 is a cell-surface sialoglycoprotein expressed by a variety of haematopoietically derived cells, including T lymphocytes. Earlier observations of defective CD43 expression by T lymphocytes from boys with the X-chromosome-linked Wiskott-Aldrich syndrome suggested the importance of CD43 in lymphocyte function. Subsequent studies have suggested that CD43 facilitates leukocyte adhesion and has a co-stimulatory role during T-cell activation. To define the physiologically relevant function(s) of CD43, we have generated CD43-knockout mice. We report here that CD43-deficient T cells from such mice show a marked increase in their in vitro proliferative response to concanavalin A, anti-CD3, the superantigen SEB and allostimulation. Additionally, CD43-deficient T cells show a substantial enhancement in homotypic adhesion and in their ability to bind different ligands, including fibronectin and the intercellular adhesion molecule ICAM-1. Vaccinia-virus-infected CD43-knockout mice mounted an augmented anti-vaccinia cytotoxic T-cell response compared with their wild-type littermates, yet developed an increased virus load. We conclude that CD43 negatively regulates T-cell activation and adhesion and is important for viral clearance.
CD43是一种细胞表面唾液酸糖蛋白，由多种造血来源的细胞表达，包括T淋巴细胞。早期观察发现，患有X染色体连锁的威斯科特-奥尔德里奇综合征的男孩的T淋巴细胞中CD43表达缺陷，这表明CD43在淋巴细胞功能中具有重要作用。随后的研究表明，CD43促进白细胞黏附，并在T细胞活化过程中具有共刺激作用。为了确定CD43的生理相关功能，我们制备了CD43基因敲除小鼠。我们在此报告，来自此类小鼠的CD43缺陷型T细胞对刀豆球蛋白A、抗CD3、超抗原SEB和同种异体刺激的体外增殖反应显著增加。此外，CD43缺陷型T细胞在同型黏附以及结合不同配体（包括纤连蛋白和细胞间黏附分子ICAM-1）的能力方面有显著增强。与野生型同窝小鼠相比，感染痘苗病毒的CD43基因敲除小鼠产生了增强的抗痘苗细胞毒性T细胞反应，但病毒载量增加。我们得出结论，CD43负向调节T细胞活化和黏附，对病毒清除很重要。
