Bellgrau D, Gold D, Selawry H, Moore J, Franzusoff A, Duke R C
Department of Immunology, University of Colorado School of Medicine, Denver 80262, USA.
Nature. 1995 Oct 19;377(6550):630-2. doi: 10.1038/377630a0.
Testis is a remarkable immune-privileged site, long known for its ability to support allogeneic and xenogeneic tissue transplants. Here we have investigated the molecular basis for testis immune privilege. Testis grafts derived from mice that can express functional CD95 (Fas or Apo-1) ligand survived indefinitely when transplanted under the kidney capsule of allogeneic animals, whereas testis grafts derived from mutant gld mice, which express non-functional ligand, were rejected. Further analysis of testis showed that CD95 ligand messenger RNA is constitutively expressed by testicular Sertoli cells, and that Sertoli cells from normal mice, but not gld mice, were accepted when transplanted into allogeneic recipients. CD95 ligand expression in the testis probably acts by inducing apoptotic cell death of CD95-expressing, recipient T cells activated in response to graft antigens. These findings indicate that CD95 ligand could be used to create immune-privileged tissue for a variety of transplant uses.
睾丸是一个显著的免疫特惠部位，长期以来因其支持同种异体和异种组织移植的能力而闻名。在此，我们研究了睾丸免疫特惠的分子基础。当从小鼠获取的能表达功能性CD95（Fas或Apo-1）配体的睾丸移植物被移植到同种异体动物的肾包膜下时，它们能无限期存活，而源自表达无功能配体的突变型gld小鼠的睾丸移植物则会被排斥。对睾丸的进一步分析表明，CD95配体信使核糖核酸由睾丸支持细胞组成性表达，并且当正常小鼠而非gld小鼠的支持细胞被移植到同种异体受体中时，它们会被接受。睾丸中CD95配体的表达可能通过诱导因移植抗原而被激活的表达CD95的受体T细胞发生凋亡性细胞死亡来发挥作用。这些发现表明，CD95配体可用于为各种移植用途创建免疫特惠组织。
