Aiba T
Department of Neurosurgery, Niigata University, Japan.
Neurol Med Chir (Tokyo). 1995 Jun;35(6):347-52. doi: 10.2176/nmc.35.347.
The characteristics of isolated and subcultured Mongolian gerbil brain microvessel endothelial cells (BECs) were investigated, especially the disappearance of blood brain barrier (BBB) functions, and subcultured BECs were co-cultured with gerbil glial cells to assess a mass-producible BBB filter model. The subcultured cells lost specific characteristics observed in freshly isolated BECs, such as limited permeability to albumin and inulin, high gamma-glutamyl transpeptidase activity, and strong connections between individual cells. The subcultured BECs did not regain BBB functions during co-culture with glial cells, even derived from the same species. Only freshly isolated endothelial cells can be used for the BBB filter model, and a mass-producible BBB filter model may be impossible to make.
对分离培养的蒙古沙鼠脑微血管内皮细胞（BECs）的特性进行了研究，尤其关注血脑屏障（BBB）功能的丧失，并将传代培养的BECs与沙鼠神经胶质细胞共培养，以评估一种可大量生产的BBB过滤模型。传代培养的细胞失去了新鲜分离的BECs所具有的特定特性，如对白蛋白和菊粉的通透性有限、高γ-谷氨酰转肽酶活性以及单个细胞之间的紧密连接。传代培养的BECs在与神经胶质细胞共培养期间（即使这些神经胶质细胞来自同一物种）也没有恢复BBB功能。只有新鲜分离的内皮细胞可用于BBB过滤模型，且可能无法建立一种可大量生产的BBB过滤模型。
