Behnisch T, Reymann K G
Federal Institute for Neurobiology, Department of Neurophysiology, Magdeburg, Germany.
Neurosci Lett. 1995 Jun 16;192(3):185-8. doi: 10.1016/0304-3940(95)11641-9.
To elucidate the role of calcium release from internal stores during different paradigms of tetanisation in long-term potentiation (LTP), we have investigated the effects of thapsigargin on the elevation of the excitatory postsynaptic field potential (fEPSP) and the population spike (PS) after tetanisation. We found no effect on the duration of fEPSP potentiation if thapsigargin was perfused before strong (triple) tetanisation. However, the potentiation was reduced significantly from control experiments if thapsigargin was applied before weak (single) tetanisation. Surprisingly, we did not find any reduction of PS potentiation, which could be due to changes in the recurrent inhibition. We conclude that the involvement of internal calcium release in the mechanisms of LTP induction will be reduced if multiple tetanisation is used.
为了阐明在长时程增强（LTP）的不同强直范式期间从内部储存库释放钙的作用，我们研究了毒胡萝卜素对强直刺激后兴奋性突触后场电位（fEPSP）和群体峰电位（PS）升高的影响。如果在强（三次）强直刺激之前灌注毒胡萝卜素，我们发现对fEPSP增强的持续时间没有影响。然而，如果在弱（单次）强直刺激之前应用毒胡萝卜素，与对照实验相比，增强作用显著降低。令人惊讶的是，我们没有发现PS增强有任何降低，这可能是由于反馈抑制的变化所致。我们得出结论，如果使用多次强直刺激，内部钙释放参与LTP诱导机制的程度将会降低。
