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[利用DNA探针的RFLP方法进行亲子鉴定]

[Paternity determination using the RFLP method in DNA probes].

作者信息

Lászik A, Hubert P, Vera M, Volkmar S

机构信息

Semmelweis Orvostudományi Egyetem, Igazságügyi Orvostani Intézet, Budapest.

出版信息

Orv Hetil. 1995 Sep 24;136(39):2117-9.

PMID:7566946
Abstract

By the discovery of restriction fragment length polymorphism and hypervariable minisatellite sequences paternity testings have become possible with almost one hundred percent probability. The procedure is based on the improvement of DNA probes which can recognize the different regions of minisatellites in the whole site of human genome. These sequences are found in noncoding regions of DNA, in the so-called intrones, in areas between genes. The sequence motives have a discriminating power and provide a possibility to distinguish individuals.

摘要

通过限制性片段长度多态性和高变微卫星序列的发现,亲子鉴定已成为可能,其准确率几乎可达百分之百。该方法基于DNA探针的改进,这些探针能够识别整个人类基因组位点中微卫星的不同区域。这些序列存在于DNA的非编码区域,即所谓的内含子中,位于基因之间的区域。序列基序具有鉴别能力,能够区分个体。

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