Niemer I, Schmelzer C, Börner G V
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Nucleic Acids Res. 1995 Sep 11;23(17):2966-72.
The group II intron bl1, the first intron of the mitochondrial cytochrome b gene in yeast is self-splicing in vitro. Genetic evidence suggests that trans-acting factors are required for in vivo splicing of this intron. In accordance with these findings, we present in vitro data showing that splicing of bl1 under physiological conditions depends upon the presence of proteins of a mitochondrial lysate. ATP is an essential component in this reaction. Overexpression of the nuclear-encoded DEAD box protein pMSS116 results in a marked increase in the ATP-dependent splicing activity of the extract, suggesting that pMSS116 may play an important role in splicing of bl1.
第二组内含子bl1是酵母线粒体细胞色素b基因的第一个内含子，它在体外能够自我剪接。遗传学证据表明，该内含子在体内剪接需要反式作用因子。根据这些发现，我们提供了体外实验数据，表明在生理条件下bl1的剪接依赖于线粒体裂解物中蛋白质的存在。ATP是该反应的必需成分。核编码的DEAD盒蛋白pMSS116的过表达导致提取物中ATP依赖的剪接活性显著增加，这表明pMSS116可能在bl1的剪接中起重要作用。
