Iacona V A, Simonetta A C, Renzulli P M
Cátedra de Microbiología General, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Rev Argent Microbiol. 1995 Jan-Mar;27(1):21-7.
The incidence of mesophilic aerobic sporulate bacteria in chicken carcasses and hamburgers was studied and the species of isolated sporulate bacteria were identified. Forty seven eviscerated carcasses from a processing plant of Entre Ríos province (Argentina) were analyzed together with fifty samples of hamburgers from two supermarkets of Santa Fe city. All carcasses resulted in contamination with aerobic mesophilic bacteria in the range from 6 x 10(3) to 1.2 x 10(6) CFU/ml liquid washed, and 94% them with sporulate bacteria, the threshold being under 100 CFU/ml (Figure 1). Hamburgers from both places resulted with aerobic mesophilic bacteria in 100% of the cases, in the range of 1 x 10(5)-3.3 x 10(6) CFU/g for supermarket A and 2.2 x 10(5) to 1.7 x 10(7) CFU/g for supermarket B; the incidence of sporulate bacteria was between 4.3 x 10(2) and 1.2 x 10(4) CFU/g for A, while the range for B was 6.2 x 10(2) and 3.8 x 10(4) CFU/g (Figure 2). Two hundred and fourteen Bacillus Genus strains were isolated and purified from the carcasses and five hundred and ninety five from hamburgers. B. subtilis and B. megaterium were most involved in carcasses; while B. licheniformis, B. subtilis and B. pumilus were in hamburgers from supermarket A and B. subtilis and B. pumilus were found in supermarket B (Table 1). The presence of B. cereus was also found, although in low levels, in all the samples (Table 1). Pollution levels with aerobic mesophilic bacteria are high in both kinds of samples.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了鸡胴体和汉堡中嗜温需氧芽孢杆菌的发生率,并对分离出的芽孢杆菌种类进行了鉴定。分析了来自恩特雷里奥斯省(阿根廷)一家加工厂的47个去内脏鸡胴体,以及来自圣菲市两家超市的50个汉堡样本。所有鸡胴体经液体冲洗后,需氧嗜温菌污染范围为6×10³至1.2×10⁶CFU/ml,其中94%含有芽孢杆菌,阈值为100CFU/ml以下(图1)。来自两地的汉堡在所有情况下均检测出需氧嗜温菌,超市A的范围为1×10⁵至3.3×10⁶CFU/g,超市B的范围为2.2×10⁵至1.7×10⁷CFU/g;超市A芽孢杆菌的发生率在4.3×10²至1.2×10⁴CFU/g之间,而超市B的范围为6.2×10²至3.8×10⁴CFU/g(图2)。从鸡胴体中分离并纯化出214株芽孢杆菌属菌株,从汉堡中分离出595株。枯草芽孢杆菌和巨大芽孢杆菌在鸡胴体中最为常见;而地衣芽孢杆菌、枯草芽孢杆菌和短小芽孢杆菌存在于超市A和B的汉堡中,枯草芽孢杆菌和短小芽孢杆菌在超市B中被发现(表1)。在所有样本中也发现了蜡样芽孢杆菌,尽管含量较低(表1)。两种样本中需氧嗜温菌的污染水平都很高。(摘要截断于250字)