Bava A J, Negroni R
Centro de Micología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay, Argentina.
Rev Inst Med Trop Sao Paulo. 1994 Nov-Dec;36(6):551-4. doi: 10.1590/s0036-46651994000600013.
The efficacy of flucytosine (5-FC) and fluconazole (FLU) association in the treatment of a murine experimental model of cryptococcosis, was evaluated. Seven groups of 10 Balb C mice each, were intraperitoneally inoculated with 10(7) cells of Cryptococcus neoformans. Six groups were allocated to receive 5-FC (300 mg/kg) and FLU (16 mg/kg), either combined and individually, by daily gavage beginning 5 days after the infection, for 2 and 4 weeks. One group received distilled water and was used as control. The evaluation of treatments was based on: survival time; macroscopic examination of brain, lungs, liver and spleen at autopsy; presence of capsulated yeasts in microscopic examination of wet preparations of these organs and cultures of brain homogenate. 5-FC and FLU, individually or combined, significantly prolonged the survival time of the treated animals with respect to the control group (p < 0.01). Animals treated for 4 weeks survived significantly longer than those treated for 2 weeks (p < 0.01). No significant differences between the animals treated with 5-FC and FLU combined or separately were observed in the survival time and morphological parameters. The association of 5-FC and FLU does not seem to be more effective than 5-FC or FLU alone, in the treatment of this experimental model of cryptococcosis.
评估了氟胞嘧啶（5-FC）与氟康唑（FLU）联合用药在小鼠隐球菌病实验模型治疗中的疗效。将七组，每组10只Balb C小鼠，腹腔接种10⁷ 新型隐球菌细胞。六组在感染后5天开始每天通过灌胃给予5-FC（300 mg/kg）和FLU（16 mg/kg），联合用药或单独用药，持续2周和4周。一组接受蒸馏水作为对照。治疗评估基于：生存时间；尸检时对脑、肺、肝和脾的宏观检查；这些器官湿片镜检和脑匀浆培养中包膜酵母的存在情况。与对照组相比，5-FC和FLU单独或联合用药均显著延长了治疗动物的生存时间（p < 0.01）。治疗4周的动物比治疗2周的动物存活时间显著更长（p < 0.01）。在生存时间和形态学参数方面，未观察到5-FC和FLU联合用药与单独用药的动物之间存在显著差异。在该隐球菌病实验模型的治疗中，5-FC与FLU联合用药似乎并不比单独使用5-FC或FLU更有效。
