Deplazes P, Guscetti F, Wunderlin E, Bucklar H, Skaggs J, Wolff K
Institut für Parasitologie, Universität Zürich.
Schweiz Arch Tierheilkd. 1995;137(5):172-9.
At their entry into the animal domicile, "La Stampa", in Lugano (canton Tessin), 217 stray dogs and 154 unwanted dogs were examined for infections with intestinal parasites, filariae, Babesia and Leishmania. The following techniques were used for detection of intestinal parasites: combined sedimentation-flotation, MIFC technique and scotch tape adherence test. Prevalences of helminth egg excretion in stray dogs and in unwanted dogs, respectively, were as follows: 34% and 22% for Trichuris, 17% and 14% for Toxocara, 3% and 5% for hookworms, 4% and 0% for taeniids. Dipylidium, Toxascaris and Capillaria were diagnosed sporadically. Samples positive for taeniids were further tested for the presence of Echinococcus coproantigens in a sandwich ELISA: one of 9 dogs was strongly positive. This dog was euthanized for security reasons and upon dissection, 11 Taenia hydatigena and more than 10,000 gravid Echinococcus granulosus worms were found. Microfilariae were detected in the blood of 3 stray dogs and one unwanted dog by the Difil-test. In all 4 cases the infective species was Dirofilaria immitis as confirmed by morphology, acid phosphatase activity analysis of microfilariae and by detection of specific antigens in blood plasma by ELISA. Specific antibodies against antigen of Leishmania infantum could not be detected in any of these dogs by ELISA. However, 3 stray dogs had specific antibodies against antigen of Babesia canis as demonstrated by IFAT.
在卢加诺(提契诺州)的“La Stampa”动物收容所,对217只流浪狗和154只弃养犬进行了肠道寄生虫、丝虫、巴贝斯虫和利什曼原虫感染检查。采用以下技术检测肠道寄生虫:沉淀-漂浮联合法、MIFC技术和透明胶带粘贴试验。流浪狗和弃养犬中蠕虫卵排泄的患病率分别如下:鞭虫为34%和22%,蛔虫为17%和14%,钩虫为3%和5%,绦虫为4%和0%。犬复孔绦虫、弓首蛔虫和毛细线虫为散发性诊断。对绦虫阳性样本进一步用夹心ELISA检测细粒棘球绦虫粪抗原的存在:9只狗中有1只为强阳性。出于安全考虑,这只狗被安乐死,解剖后发现11条泡状带绦虫和10000多条成熟的细粒棘球绦虫。通过Difil试验在3只流浪狗和1只弃养犬的血液中检测到微丝蚴。在所有4例中,经形态学、微丝蚴酸性磷酸酶活性分析以及ELISA检测血浆中特异性抗原证实,感染种均为犬恶丝虫。通过ELISA在这些狗中均未检测到针对婴儿利什曼原虫抗原的特异性抗体。然而,3只流浪狗通过间接荧光抗体试验显示出针对犬巴贝斯虫抗原的特异性抗体。