Toda Y, Wada T, Morimoto T, Minamikawa Y, Akagi S, Sugano H, Mori Y, Nishimura H, Arita S, Sugino Y
Department of Orthopaedic Surgery, Kansai Medical University Otokoyama Hospital, Kyoto.
Ryumachi. 1995 Jun;35(3):514-20.
The relationship between genotypes of HLA-DRB 1 alleles and the progression of bone destruction of RA patients was assessed. The genotypes of the HLA-DRB 1 alleles were determined in 329 Japanese patients with seropositive RNA by polymerase chain reaction and allele-specific oligonucleotide probe techniques. We regarded HLA-DRB 1* 0101, 0401, 0404, 0405, 1001 and 1402 as susceptible alleles of RA and classified the patients into three groups. The s/s group consisted of those with susceptible factors in both of the HLA-DRB 1 alleles. The s/n group was made up of those having one susceptible factor and one non-susceptible factor. The n/n group consisted of those possessing two non-susceptible factors. The grading of radiographic change that was evaluated by Larsen's criteria compared with the genotyped results. In the result, the median years taken to development to grade III, IV and V were significantly shorter in the s/s groups compared with that in the s/n and n/n groups. Thus, genotyping of HLA-DRB 1 can be a useful prognostic market in the progression of bone destruction of RA.
评估了HLA - DRB 1等位基因的基因型与类风湿关节炎（RA）患者骨破坏进展之间的关系。采用聚合酶链反应和等位基因特异性寡核苷酸探针技术，对329例血清反应阳性的日本RA患者的HLA - DRB 1等位基因进行基因分型。我们将HLA - DRB 1*0101、0401、0404、0405、1001和1402视为RA的易感等位基因，并将患者分为三组。s/s组由两个HLA - DRB 1等位基因均有易感因素的患者组成。s/n组由有一个易感因素和一个非易感因素的患者组成。n/n组由具有两个非易感因素的患者组成。根据Larsen标准评估的影像学变化分级与基因分型结果进行比较。结果显示，与s/n组和n/n组相比，s/s组发展到III级、IV级和V级的中位时间显著缩短。因此，HLA - DRB 1基因分型可作为RA骨破坏进展的有用预后指标。
