Wilsher M L, Hallowes M, Birchall N M
Department of Respiratory Medicine, Green Lane Hospital, Auckland 3, New Zealand.
Thorax. 1995 Aug;50(8):858-62. doi: 10.1136/thx.50.8.858.
Gamma/delta T lymphocytes are thought to have a role in granulomatous immune responses at peripheral sites of antigen contact such as the gut, skin and lung. The aim of this study was to determine if gamma/delta T lymphocytes are increased in the peripheral blood of patients with active sarcoidosis.
Peripheral blood from 21 untreated patients with a new presentation of sarcoidosis (12M, 9F), 20 normal volunteers (12M, 8F), and 12 patients with cavitary pulmonary tuberculosis were subjected to Ficoll Hypaque separation and flow cytometry analysis using monoclonal antibodies to CD3, 4, 8, 25, HLA-DR and gamma/delta T cell receptor.
All patients with sarcoidosis had compatible chest radiographs and all were Mantoux negative in spite of previous BCG vaccination. In all but one patient histological examination showed non-caseating granuloma. There was no difference in the mean percentage or absolute numbers of gamma/delta positive peripheral blood lymphocytes between the three populations. Thirteen patients with sarcoidosis had an absolute lymphopenia and the mean percentage of CD3 positive peripheral blood lymphocytes in the group with sarcoidosis was lower than the other two groups. The percentage of CD25 and HLA-DR positive cells was higher in the group with sarcoidosis, supporting the fact that these patients had active disease.
Gamma/delta T lymphocytes are not increased in the peripheral blood of patients with sarcoidosis and are unlikely to have a role in the pathogenesis of this disease.
γ/δ T淋巴细胞被认为在抗原接触的外周部位如肠道、皮肤和肺部的肉芽肿性免疫反应中发挥作用。本研究的目的是确定活动性结节病患者外周血中γ/δ T淋巴细胞是否增多。
对21例新诊断为结节病的未治疗患者（12例男性，9例女性）、20名正常志愿者（12例男性，8例女性）和12例空洞型肺结核患者的外周血进行Ficoll-Hypaque分离，并使用抗CD3、4、8、25、HLA-DR和γ/δ T细胞受体的单克隆抗体进行流式细胞术分析。
所有结节病患者的胸部X线片均符合诊断，尽管之前接种过卡介苗，但所有患者结核菌素试验均为阴性。除1例患者外，所有患者的组织学检查均显示非干酪样肉芽肿。三组人群中γ/δ阳性外周血淋巴细胞的平均百分比或绝对数量无差异。13例结节病患者存在绝对淋巴细胞减少，结节病组外周血CD3阳性淋巴细胞的平均百分比低于其他两组。结节病组CD25和HLA-DR阳性细胞的百分比更高，这支持了这些患者患有活动性疾病这一事实。
结节病患者外周血中γ/δ T淋巴细胞未增多，不太可能在该疾病的发病机制中起作用。
