大麦轻度花叶病毒真菌传播和机械传播分离株的特性：两种毒株的竞争关系

Characterization of fungally and mechanically transmitted isolates of barley mild mosaic virus: two strains in competition.

作者信息

Dessens J T, Meyer M

机构信息

Station de Pathologie Végétale, Institut National de la Recherche Agronomique, Versailles, France.

出版信息

Virology. 1995 Oct 1;212(2):383-91. doi: 10.1006/viro.1995.1495.

DOI:10.1006/viro.1995.1495

PMID:7571407

Abstract

The virus populations of two French barley mild mosaic virus isolates have been characterized using Western blot, sequence, PCR, and RFLP analyses. One of the isolates (M) was obtained by mechanical transmission of the other, fungally transmitted isolate (P). The results obtained show that the two isolates contain distinct RNA1 and RNA2 molecules with respect to their nucleotide sequences, and hence contain distinct barley mild mosaic virus strains. One strain is present mainly in isolate P and closely resembles a Japanese and two German isolates. It contains an RNA2 of approximately 3.5 kb. The other strain is present mainly in isolate M and closely resembles a UK isolate. This strain contains a smaller RNA2 of approximately 2.4 kb. The results show that mechanical transmission causes a shift in the virus populations in favor of the mutant strain.

摘要

利用蛋白质免疫印迹法、测序法、聚合酶链式反应（PCR）以及限制性片段长度多态性分析（RFLP）对两种法国大麦轻度花叶病毒分离株的病毒群体进行了特征分析。其中一种分离株（M）是通过机械传播另一种真菌传播的分离株（P）获得的。所得结果表明，就核苷酸序列而言，这两种分离株含有不同的RNA1和RNA2分子，因此含有不同的大麦轻度花叶病毒株系。一种株系主要存在于分离株P中，与一种日本分离株和两种德国分离株非常相似。它含有一条约3.5 kb的RNA2。另一种株系主要存在于分离株M中，与一种英国分离株非常相似。该株系含有一条约2.4 kb的较小RNA2。结果表明，机械传播导致病毒群体向有利于突变株系的方向转变。