Kamal M M, Luley A S, Mundhada S G, Bobhate S K
Department of Pathology, Government Medical College, Nagpur, Maharashtra, India.
Acta Cytol. 1995 Sep-Oct;39(5):931-5.
To determine the utility of the simple technique of scrape cytology for obtaining a morphologic diagnosis of Rhinosporodium seeberi.
The study included nine patients presenting with a nasal polyp alone and one patient presenting with a skin ulcer with a recurrent nasal polyp. Scrape smears from all 10 nasal polyps and skin ulcers were collected. Periodic acid-Schiff (PAS) stain was used on smears from three cases. Histopathology of all the nasal polyps and the excised skin ulcer was done.
The scrape smears showed characteristic spores of R seeberi mixed with an acute and chronic inflammatory exudate and metaplastic cells. PAS stain showed positivity for the capsule. Histopathology confirmed the cytologic diagnosis.
Since mature sporangia rupture either on the surface or within the mucosa of polyps, scrape smears reliably and safely retrieve abundant spores. Therefore, this technique, offers immense potential for a rapid and correct preoperative diagnosis of rhinosporodiosis. It thereby facilitates the planning of surgical interventions to prevent recurrences.
确定刮片细胞学这一简单技术在获得西伯鼻孢子虫形态学诊断方面的效用。
该研究纳入了9例仅患有鼻息肉的患者和1例患有皮肤溃疡并伴有复发性鼻息肉的患者。收集了所有10个鼻息肉和皮肤溃疡的刮片。对3例的涂片进行了过碘酸希夫（PAS）染色。对所有鼻息肉和切除的皮肤溃疡进行了组织病理学检查。
刮片显示西伯鼻孢子虫的特征性孢子与急性和慢性炎性渗出物及化生细胞混合存在。PAS染色显示荚膜呈阳性。组织病理学证实了细胞学诊断。
由于成熟的孢子囊在息肉表面或黏膜内破裂，刮片能可靠且安全地获取大量孢子。因此，该技术在快速、正确地进行鼻孢子虫病术前诊断方面具有巨大潜力。从而有助于规划手术干预措施以预防复发。
