Nissen A K, Lehn-Jensen H, Hyttel P, Greve T
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Acta Vet Scand. 1995;36(1):123-33. doi: 10.1186/BF03547709.
Follicular growth, chronology of ovulation and embryo morphology were compared in sows ovulating spontaneously and sows, in which the ovulation was attempted induced by hCG or GnRH. Indwelling catheters were placed on day 1 (weaning = day 0) in the ear veins of 18 sows, which were then randomly divided into 3 groups: a control group (N = 6), a group (N = 6) given 750 iu hCG (Physex) im 76h after weaning (hCG group) and a group (N = 6) given 500 micrograms GnRH (Fertagyl) im 76h (N = 3) or 100h after weaning (N = 3) (GnRH group). Follicular diameter and time of ovulation were monitored by ultrasonography every 4h from day 3 until ovulation or development of cysts by means of a sector scanner fitted with a 5.0/7.5 MHz multiangle probe. Heat detection was performed every 8h from day 3 until ovulation. On day 13, the sows were slaughtered, the number of corpora luteae (CL) was counted, and embryos were flushed from the uteri. The control group showed clear heat symptoms, and on day 3, the follicles were typically 3-7 mm and grew up to 7-10 mm over 2 days, where they remained for approximately 24h until ovulation took place 41h +/- 9h after first sign of standing heat. The hCG group exhibited no signs of heat, and the follicles only reached 5-8 mm in diameter at time of ovulation, which occurred 40h +/- 1h after hCG-injection. The GnRH group exhibited inconsistent signs of heat, and the follicles reached a maximum size of 7-12 mm in diameter where they remained for more than 24h. Only 2 sows in this group ovulated within 84-92h after the GnRH injection, and development of bursa cysts and cystic follicles was a common finding. The average number of CL was 18.2 +/- 5.7 per sow (N = 16, range: 3-27) with no significant difference between the groups. Total embryo recovery was 79 +/- 13% with no significant difference between groups. The embryo diversity calculated as standard deviation of the maximum diameter was higher in the hCG group as compared with the control group.(ABSTRACT TRUNCATED AT 400 WORDS)
对自然排卵的母猪以及尝试用绒毛膜促性腺激素（hCG）或促性腺激素释放激素（GnRH）诱导排卵的母猪的卵泡生长、排卵时间和胚胎形态进行了比较。在第1天（断奶日=第0天），将留置导管置于18头母猪的耳静脉中，然后将其随机分为3组：对照组（N = 6）、在断奶后76小时肌肉注射750国际单位hCG（Physex）的组（N = 6）（hCG组）以及在断奶后76小时（N = 3）或100小时（N = 3）肌肉注射500微克GnRH（Fertagyl）的组（N = 6）（GnRH组）。从第3天开始，每隔4小时通过配备5.0/7.5 MHz多角度探头的扇形扫描仪，用超声检查监测卵泡直径和排卵时间，直至排卵或囊肿形成。从第3天开始，每隔8小时进行一次发情检测，直至排卵。在第13天，宰杀母猪，统计黄体数量，并从子宫中冲洗出胚胎。对照组出现明显的发情症状，在第3天，卵泡通常为3 - 7毫米，在2天内长到7 - 10毫米，在那里停留约24小时，直到在站立发情的第一个迹象出现后41小时±9小时排卵。hCG组没有发情迹象，排卵时卵泡直径仅达到5 - 8毫米，在注射hCG后40小时±1小时排卵。GnRH组发情迹象不一致，卵泡直径最大达到7 - 12毫米，在那里停留超过24小时。该组只有2头母猪在注射GnRH后84 - 92小时内排卵，囊状囊肿和卵泡囊肿的形成很常见。每头母猪黄体的平均数量为18.2±5.7个（N = 16，范围：3 - 27），各组之间无显著差异。胚胎总回收率为79±13%，各组之间无显著差异。以最大直径的标准差计算的胚胎多样性，hCG组高于对照组。（摘要截断于400字）
