Serkedjieva J
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Acta Virol. 1995 Feb;39(1):5-10.
A polyphenolic complex (PC) with antiviral properties has been isolated from the Bulgarian medicinal plant Geranium sanguineum L. A study was undertaken to investigate the effect of PC on virus-specific protein synthesis in influenza virus-infected cells. The expression of viral glycoproteins on the surface of chick embryo fibroblasts infected with virus A/FPV, strain Rostock (H7N1) was suppressed. Virus protein synthesis was selectively inhibited as shown by SDS polyacrylamide gel electrophoresis of 35S-methionine-labelled proteins and proteins immunoprecipitated with monoclonal antibodies. The inhibitory effect was dose-dependent and better pronounced when PC was applied after virus infection. Two variants of influenza virus FPV/Rostock with reduced drug susceptibility were selected. PC affected to a lesser extent the synthesis of viral proteins in cells infected with the variants as compared to the sensitive parental virus.
一种具有抗病毒特性的多酚复合物（PC）已从保加利亚药用植物血红老鹳草中分离出来。开展了一项研究以调查PC对流感病毒感染细胞中病毒特异性蛋白质合成的影响。感染了罗斯托克株（H7N1）A/FPV病毒的鸡胚成纤维细胞表面病毒糖蛋白的表达受到抑制。如用35S-甲硫氨酸标记的蛋白质以及用单克隆抗体免疫沉淀的蛋白质进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳所示，病毒蛋白质合成受到选择性抑制。抑制作用呈剂量依赖性，且在病毒感染后应用PC时更为明显。选择了两种对药物敏感性降低的流感病毒FPV/罗斯托克变异株。与敏感的亲本病毒相比，PC对感染变异株的细胞中病毒蛋白质合成的影响较小。
